摘要
以7年生糯米糍荔枝茎段脱分化形成的愈伤组织为材料,对愈伤组织的形态和结构进行观察。结果表明,荔枝茎段愈伤组织的增殖缓慢,分化非常困难,主要分为结构疏松、颗粒状、柱状突起和结构致密等4种类型。其中,颗粒状和柱状突起的愈伤组织细胞质浓,细胞核大,分生潜力相对较强,但增殖较慢,它们在MS+2,4-D 2.0mg/L+BA 0.5mg/L+CH 500mg/L+PVP 2.0g/L培养基上诱导后得到了早期阶段的球形胚。
The morphology and structure of callus from stem segments of 7-year-old Litchi were ob- served. The Results showed that the proliferation of Litchi callus was very slow and the differentia- tion of the callus was difficult. There were 4 types of callus： loose structured, granule, pillar ena- tion, and compacted structure. The cells of granule and pillar enation callus had dense cytoplasm and large nucleus, and had the best, though slow in proliferation, differentiation capability. When callus were cultured on MS＋2,4-D 2. 0 mg/L＋BA 0. 5 mg/L＋CH 500 mg/L＋PVP 2. 0 g/L, early globular embryo appeared.
出处
《中国南方果树》
北大核心
2012年第4期25-27,32,共4页
South China Fruits
