摘要
利用RT-PCR技术从溆浦鹅卵泡的颗粒细胞总RNA中扩增出了抑制素α亚基成熟区序列,经RCR扩增出354bp片段,该片段与pMD 18T载体连接,转化JM109感受态细胞,所得阳性克隆进行双酶切鉴定和PCR鉴定,并进行了测序分析,得到的克隆序列与设计的序列基本一致。表明已成功地获得了抑制素α亚基编码序列的克隆载体。
Maturation zone of inhibin αsubunit was amplified from granular cell of Xüpu goose ovarian follicle by RT-PCR technology. The recombinant plasmid pMD-18T-IB was constructed by inserting the fragment of 354 bp obtained by PCR into pMD-18T vector. The sense clone from JM109 cell was evaluated by double enzyme digestion and PCR. DNA sequence analysis showed that the cloned sequence was the very fragment designed. The clone vector of Xüpu goose obtained was inhibin αsubunit.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2004年第1期89-92,共4页
Journal of Jilin Agricultural University
基金
吉林省自然科学基金资助项目(20020654)
