摘要
Inhibin was purified from swine ovarian fluid by deposition and column deposition.The purified inhibin was diluted with ureasodiumacetate,and then was mixed with Freund adjuvant with same volume.Each of the twenty goats was given subcutaneous injections of the purified inhibin 2 mg at neck.The goats were given boosterinjections of the inhibin 1 mg that was mixed with ureasodiumacetate and Freund incomplete adjuvant after 30 and 50 days.Five goats in control group were given admixture of urea and sodium acetate and adjuvant at the same time.The goats were injected with prostaglandin F2a 005 mg at 21 d and 31 d after the last immunization.The blood samples were collected at 30 d,51 d and 60 d after the first immunization and before immunization.The plasma FSH concentrates was assayed with RIA and the inhibin antibody titer was assayed with agar plate diffusion method.The plasma antibody titer was 1∶16256 at 60 d after first immunization.The plasma FSH concentrates of the immunized goats was higher than those of the control group(202 vs.12 mIU/ml,PZ<005).The number of follicle and the number of corpora lutea of the immunized group were as twice as those of the control(1931 vs.1223 and 225 vs.110,respectively).It indicated that inhibin purified from swine ovarian fluid could induce goat produce high ovulation rate and it can improve reproductive traits of goat.
Inhibin was purified from swine ovarian fluid by deposition and column deposition.The purified inhibin was diluted with ureasodiumacetate,and then was mixed with Freund adjuvant with same volume.Each of the twenty goats was given subcutaneous injections of the purified inhibin 2 mg at neck.The goats were given boosterinjections of the inhibin 1 mg that was mixed with ureasodiumacetate and Freund incomplete adjuvant after 30 and 50 days.Five goats in control group were given admixture of urea and sodium acetate and adjuvant at the same time.The goats were injected with prostaglandin F2a 005 mg at 21 d and 31 d after the last immunization.The blood samples were collected at 30 d,51 d and 60 d after the first immunization and before immunization.The plasma FSH concentrates was assayed with RIA and the inhibin antibody titer was assayed with agar plate diffusion method.The plasma antibody titer was 1∶16256 at 60 d after first immunization.The plasma FSH concentrates of the immunized goats was higher than those of the control group(202 vs.12 mIU/ml,PZ<005).The number of follicle and the number of corpora lutea of the immunized group were as twice as those of the control(1931 vs.1223 and 225 vs.110,respectively).It indicated that inhibin purified from swine ovarian fluid could induce goat produce high ovulation rate and it can improve reproductive traits of goat.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2003年第1期37-39,共3页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
江苏省科技厅(BJ99040)
国家自然科学基金(3007055)资助
关键词
猪卵泡抑制素
主动免疫
山羊
生殖
Inhibin
Active immunization
Ovulation Rate
FSH
Goat
