摘要
使用酶法、酸法和碱法分别进行苦荞麦蛋白质提取试验。结果证明采用碱性蛋白酶法提取工艺效果最好,最佳的工艺条件为温度35℃、酶的用量为50U/g、料水比1:12、pH=11、反应时间30min,迅速灭酶,在pH为4.2的溶液中进行沉淀,去离子水洗,最后经过喷雾干燥即可得苦荞麦蛋白质固体粉末,蛋白质的提取率为76%,其固体粉末的蛋白质含量为68%。
Buckwheat is a valuable crop. In order to develop buckwheat into nurture and material medical, etc, we study on the extraction of buckwheat protein. From our experiment, we can draw that alkaline proteinase is the best of the used proteinase,and the optimum process is: temp.35℃ ; the ratio of material and water 1:12; alkaline proteinase 50U/g; pH=11 and extract within 30 min, extinguish proteinase quickly, after that precipitation at pH=4.2, deion- water washed, last after spray- dry, we can get BWPE(buckwheat protein extract) powder with extraction rate 76% ,and the protein content of powder is 68% .
出处
《粮食加工》
2004年第1期55-59,共5页
Grain Processing
