摘要
目的 :探讨冷哮丸对小鼠免疫功能的作用机制。方法 :采用 1 %二硝基氟苯 (DNFB)诱导小鼠DTH ,称重胸腺、脾脏及计算耳肿胀度 ,测定DTH小鼠腹腔巨噬细胞 (M)产生白细胞介素 - 1 (IL - 1 )及血清IL - 1水平 ,计数溶血空斑 (HP)水平。结果 :冷哮丸全方及拆方 1组对DNFB诱导小鼠DTH胸腺、脾脏重量及耳肿胀度有非常显著作用 ;拆方 2亦有显著的作用。全方及拆方 1组能抑制其腹腔M产生IL - 1 ;拆方 2组无明显作用。HP计数显示全方降低最明显 ,拆方 1次之 ,拆方 2组作用不明显。结论 :冷哮丸有抑制小鼠DTH作用 ,其机理可能是抑制了IL - 1等细胞因子的产生和分泌 ,从而抑制许多炎性介质如组织胺等的释放而减少粘膜水肿与减轻气道炎症。抑制小鼠HPA表明其对特异性免疫功能具有抑制作用。
Objective:In order to search the mechanism of immune function of extract of lengxiao pill in the mice.Methods:The author used the mouse delayed-type hypersensitivity(DTH)model induced by 1 percent dinitrofluorobenzene(DNFB)to weigh the spleen and thymus gland and count the tumefaction degree of ears and measure IL-l which peritoneal macrophage produces and the level of IL-l in the serum and count haemolytic plaque(HP)by DTH mice:Results:The results showed that the three prescription could inhibit DTH in mice induced by DNFB,also could decrease the weight of spleen and thymus gland,the tumefaction degree of ears and the level of IL-l in serum of DTH mice,could inhibit its peritoneal macrophage producing IL-l.And the whole recipe and isolated recipe lcould decrease HP but isolated recipe 2 could not.Conclusion:All these facts suggest that lenxiao pill has a function of suppressing the mice DTH induced by DNFB,by means of inhibiting production and secretion of the cytodines,such as IL-l et.to suppress immunologic reaction lessen immunologic pathologic lesion.As a result the inflammation of air passage can be decreased.
出处
《中医药学报》
CAS
2004年第1期14-15,共2页
Acta Chinese Medicine and Pharmacology
