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LH2中类胡萝卜素为链孢红素的类球红细菌绿色突变株(GM309)的获得及理化分析 被引量:5

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摘要 用甲基磺酸乙酯诱变类球红细菌(Rhodobacter sphaeroides 601),分离获得绿色突变株,命名为GM309。吸收光谱分析表明其外周天线色素复合物(LH2)的类胡萝卜素特征吸收峰发生了20nm的蓝移。通过吸收光谱和质谱进一步的分析确定RS601的LH2中类胡萝卜素为球形烯,而GM309的LH2中则变为链孢红素。链孢红素比球形烯少一个共轭双键。CD,吸收光谱分析表明GM309 LH2中色素排列结构与野生LH2相近。比较野生和突变株的LH2荧光发射光谱,发现GM309的LH2仍保持了激发能由类胡萝卜素向细菌叶绿素以及细菌叶绿素B800向B850的传递,其B850的荧光峰为对照的58%,表明激发能由细菌叶绿素B800向B850的传递效率有所降低。研究为了解类胡萝卜素在LH2激发能传递中的作用提供了新的实验手段。
出处 《中国科学(C辑)》 CSCD 北大核心 2003年第5期385-390,共6页 Science in China(Series C)
基金 国家重点研究发展规划(批准号:G1998010100) 国家自然科学基金(批准号:30270347)
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