摘要
目的研究金欣口服液对呼吸道合胞病毒(RSV)感染的BALB/c小鼠肺组织中TLR3信号转导通路负调控因子SOCS1不同时间点的表达趋势的调控作用。方法 75只BALB/c小鼠随机分为正常组、模型组、利巴韦林组、金欣高剂量组和金欣等效剂量组,每组15只。每组分干预方式Ⅰ、Ⅱ、Ⅲ,每种干预方式5只小鼠。RSV滴鼻感染BALB/c小鼠制备动物模型,予相应干预方式处理后处死取肺组织。Real time PCR分别检测RSV-M、SOCS1和IFN-β在mRNA水平的表达情况,并采用Western blotting检测SOCS1在蛋白水平上的表达情况。结果与正常组比较,模型组干预方式Ⅰ、ⅡSOCS1、IFN-βmRNA表达及SOCS1蛋白表达升高(P<0.01),干预方式Ⅲ时IFN-βmRNA表达降低(P<0.01)。与模型组比较,金欣高、等效剂量组3种干预方式RSV-M mRNA表达均降低(均P<0.01),金欣高剂量组干预方式Ⅰ、Ⅲ及金欣等效剂量3种干预方式SOCS1 mRNA表达降低(P<0.01);金欣高剂量组干预方式Ⅰ、Ⅱ及金欣等效剂量组干预方式ⅠIFN-βmRNA的表达降低(P<0.01),而金欣高剂量组和等效剂量组干预方式ⅢIFN-βmRNA的表达升高(P<0.01);金欣高剂量组干预方式Ⅰ及金欣等效剂量组的3种干预方式SOCS1蛋白表达明显降低,金欣高剂量组干预方式ⅢSOCS1蛋白表达升高。与金欣高剂量组比较,金欣等效剂量组干预方式Ⅰ、ⅡRSVM mRNA表达降低(P<0.01);金欣等效剂量组干预方式ⅠSOCS1 mRNA表达降低(P<0.01);金欣等效剂量组干预方式ⅠIFN-βmRNA的表达降低(P<0.01),但干预方式Ⅱ、ⅢIFN-βmRNA的表达升高(均P<0.01);金欣等效剂量组3种干预方式SOCS1蛋白表达降低。结论金欣口服液等效剂量组在不同时间点均能抑制RSV感染BALB/c小鼠肺组织中SOCS1的表达,该调控作用可能与促进Ⅰ型干扰素表达从而发挥抗RSV作用有一定关系。
Objective To investigate the regulation trend of Jinxin Oral Liquid( JXOL) on the expression of negative regulatory factor of TLR3 signaling pathway SOCS1 in the lung tissue of RSV infected BALB / c mice at different time points.Methods Totally 75 BALB / c mice were randomly divided into 5 groups,i. e.,the normal control group,the model group,the ribavirin group,the high dose JXOL group,and the equivalent dose JXOL group,15 in each group. Each group had 3 intervention ways( Ⅰ,Ⅱ,and Ⅲ) with 5 mice treated in each group. BALB / c mice were nasally infected with respiratory syncytial virus( RSV),and treated by different intervention ways. After intervention,mice were killed and their lung tissues were sampled. mRNA expression levels of RSV- M,SOCS1,and IFN- β were detected by Real time PCR. The expression of SOCS1 at the protein level was detected by Western blot. Results Compared with the normal control group,the mRNA ex-pression level of SOCS1 and IFN- β,and the protein expression level of SOCS1 increased significantly in the model group intervened by intervention Ⅰ and Ⅱ( all P < 0. 01),but the mRNA expression level of IFN- β decreased significantly in model group intervened by intervention Ⅲ(P < 0. 01). Compared with the model group,the mRNA expression level of RSV-M all significantly decreased in the high dose JXOL group and the equivalent dose JXOL group intervened by 3 intervention ways( all P < 0. 01). The mRNA expression level of SOCS1 significantly decreased in the high dose JXOL group intervened by intervention Ⅰ and Ⅲ and the equivalent dose JXOL group intervened by 3 intervention ways( all P < 0. 01). The mRNA expression level of IFN- β significantly decreased in the high dose JXOL group intervened by intervention Ⅰ and Ⅱand the equivalent dose JXOL group intervened by intervention Ⅰ( all P < 0. 01),while it significantly increased in the high dose JXOL group intervened by intervention Ⅲ and the equivalent dose JXOL group intervened by intervention Ⅲ( all P < 0. 01). The pr
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2014年第12期1499-1506,共8页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81072840)
