摘要
目的 评价转基因大米和非转基因大米对小鼠免疫功能的异同。方法 以转豇豆胰蛋白酶抑制剂 (cowpeatrypsininhibitor ,CpTI)基因大米和母系非转基因大米喂养BALB C小鼠 30天 (均配成AIN 93G的全价营养饲料 ,并且含有最大量的转基因大米或非转基因大米 ) ,比较各组小鼠的各项免疫毒理指标 :体重、脏器指数、血常规、淋巴细胞分类、血清抗体滴度、空斑试验 (PFC)、迟发型皮肤过敏反应 (DTH)、腹腔巨噬细胞吞噬鸡红细胞试验。结果 发现转基因大米和非转基因大米喂养的小鼠各项免疫毒理指标无差异。结论 转基因大米对小鼠的免疫功能的影响与非转基因大米基本相同 ,转基因大米与非转基因大米在免疫毒理学方面的评价具有“实质等同性”。
Objective To assess the immunotoxicologic aspect of transgenetic plant.Methods BALB/C mice were fed with food composed by transgenic rice (into which cowpea trypsin inhibitor gene was introduced) or nontransgenetic rice (which has the same gene composition as the transgenic rice except for the cowpea trypsin inhibitor gene ) for 30 days.All food is made according to the composition of AIN-93G.In the end,all kinds of immunotoxicologic indexes of mice of every group were compared such as body weight,guts index,blood routine test,lymphocyte sort,serum antibody titter, plaque forming cell,delayed hypersensitivity response,macrophage function test. Results All immunotoxicologic indexes of mice fed either by transgenic rice or nontransgenetic rice have no differences to those of mice fed by normal food.Conclusion Transgenic rice is substantially equivilent to nontransgenetic rice in immunotoxicologic aspect.
出处
《卫生研究》
CAS
CSCD
北大核心
2004年第1期77-80,共4页
Journal of Hygiene Research
基金
国家科技部 973计划 (No.2 0 0 1CB10 90 0 7)
国家科技部 86 3计划 (No .AA2 2 12 2 91)
科技部国家转基因植物研究与产业化专项 (No .J0 0 C 0 0 3)
关键词
转基因大米
免疫毒理学评价
实质等同性
transgenetic rice, immunotoxicologic assessment, substantial equivilence
