摘要
为研究S86转基因大米的致敏性 ,用BN大鼠致敏动物模型进行研究。 4 0只BN大鼠随机分为 4组 ,每组 10只 ,分别为 :阳性对照组 ,转基因大米组 ,非转基因大米组及阴性对照组。分别在第 0和第 7天 ,通过腹腔注射给予阳性对照组的大鼠 0 1mg ml卵清蛋白 (OVA) 1ml ,其它各组在相同时间腹腔注射相同剂量的生理盐水。以AIN - 93G为依据配制各组饲料 ,阳性对照组和阴性对照组大鼠饲以AIN - 93G饲料 ,转基因大米组饲以含转基因大米粉的饲料 ,非转基因大米组饲以含非转基因大米粉的饲料 ,时间为 6周。在第 2次腹腔注射OVA后 ,取血分离血浆进行组胺测定。分别在第 14、2 1、2 8、35、4 2天取血分离血清 ,测定IgG及IgE水平 ;此外 ,分别在实验前和实验后测定各组大鼠的血压。血清学实验结果显示转基因大米全食品喂饲没有激发IgG及IgE反应 ,而OVA(阳性对照组 )激发了明显的IgG及IgE反应 ;转基因大米组的组胺水平与阴性对照组及亲本对照组相比差异没有显著性 ,而阳性对照组组胺水平升高。另外转基因大米没有引起大鼠血压升高。该研究结果表明通过全食品喂饲的方式给予BN大鼠S86转基因大米没有发现该转基因大米对BN大鼠具有致敏性。
To assess the allergenicity of the genetically modified (GM) rice S86,4 groups of BN rats (10 for each group)were fed with different experimental diets for six weeks.A positive control group and a negative control group were fed with standard AIN-93G diet;the GM group was fed with AIN-93G-based diet containing GM rice 78.3%;the nonGM group was fed with AIN-93G-based diet containing nonGM rice 74.7%. On day 0 and day 7,rats in the positive control group were given Ovalbumin(OVA) 100 μg(1ml) intraperitoneally and rats in the other 3 groups were given normal saline of the same volume.On day 7,immediately after the intraperitoneal injection,blood samples were obtained for determination of the plasma level of histamine.On days 14,21,28,35,42,blood samples were drawn and analyzed for the serum levels of lgG and lgE.Blood pressure of the rats was measured before and after the experimental period.The results showed that no lgG or lgE response was elicited in rats of the GM rice group,while strong lgG and lgE responses were observed in rats of the positive control group;and the rats of the positive control group had significantly higher plasma level of histamine than the other 3 groups with similar histamine level.An oral challenge with OVA caused a temporary drop of the systolic pressure in 2 rats of the positive control group,and no change in blood pressure was observed in rats fed with GM rice.It is concluded that GM rice S86 has no allergenic effect on BN rats.
出处
《中国食品卫生杂志》
2005年第1期7-9,共3页
Chinese Journal of Food Hygiene
基金
国家高技术研究发展计划"863"(2 0 0 1AA2 12 2 91
2 0 0 2AA212 0 41)
国家重点基础研究发展规划"973"(0 0 1CB10 90 0 7)~~
