摘要
为探讨长期饲喂转基因大豆GTS40-3-2对雄性大鼠器官和生殖机能的影响,本研究将雌、雄大鼠随机分为2组,分别饲喂含转基因和非转基因大豆成分的饲料90 d后,亲代大鼠组内交配获得子代大鼠;子代雄性大鼠断乳后继续饲喂对应饲料30、60和90 d后,摘取子代大鼠睾丸、附睾、脑、心脏、肺脏、肝脏、脾脏、肾脏和肾上腺,检测脏器系数;选取睾丸、附睾、肾、肺制备石蜡切片,观察各器官的组织结构变化;运用实时荧光定量PCR检测睾丸雄性激素合成限制性蛋白葡萄糖转运蛋白8(GLUT8)基因的表达变化;用蛋白酶联免疫反应法进一步验证GLUT8蛋白含量。结果表明,与对照组相比,饲喂30、90 d后9种器官脏器系数均无显著差异(P>0.05);饲喂60 d后,仅肺、附睾脏器系数存在显著差异(P<0.05)。组织切片镜检结果显示,睾丸、附睾、肾、肺未发现明显结构改变;睾丸GLUT8基因转录水平和蛋白水平均未受到显著影响(P>0.05)。综上所述,转基因大豆GTS40-3-2饲料在试验周期内未对子代雄性大鼠各重要器官产生不良影响,对雄性大鼠生殖器官及其生理机能未显示毒性作用。本研究结果进一步拓展了转基因大豆安全评价研究深度,对转基因大豆的推广应用具有重要意义。
To provide a theoretical basis for the safety evaluation and promotion of genetically modified(GM)soybean,we studied the effects of GM soybean GTS40-3-2 on the organ and reproductive functions of male rats.In this study,female and male rats were randomly divided into two groups and fed diets containing GM or non-GM soybean components for 90 d,respectively.Then by mating within the parental rat groups,the offspring rats were obtained,which fed the corresponding feed after weaning.After 30,60 and 90 d of feeding,the testes,epididymis,brain,heart,lungs,liver,spleen,kidneys and adrenal glands of the offspring rats were extracted and their organ coefficients were examined;paraffin sections of testes,epididymis,kidneys and lungs were prepared,respectively,to observe the histological changes of each organ;the expression changes of glucose transporter 8(GLUT8)gene,a restriction protein for androgen synthesis in testes,were examined by real-time PCR and further verified by enzyme-linked immunosorbent assay(ELISA).The results showed that there was no significant difference in the organ coefficients of the nine organs after 30 d and 90 d feeding compared with the control group(P>0.05);after 60 d feeding,only the organ coefficients of lung and epididymis were significantly different(P<0.05),but there was no biological significance;there was no significant structural changes observed in testis,epididymis,kidney and lung by microscopic examination of tissue sections;the transcript level and protein level of GLUT8 gene in testis were not significantly affected(P>0.05).These results revealed that the feed containing GM soybean GTS40-3-2 had no any adverse effects on the vital organs of male offspring rats during the experimental period,and also on male reproductive organs and their physiological functions.This study further expanded the research depth of safety evaluation of GM soybean,which was of great significance to the popularization and application of GM soybean.
作者
史宗勇
刘璇
路超
郭俊佩
许冬梅
赵娟丽
袁建琴
SHI Zongyong;LIU Xuan;LU Chao;GUO Junpei;XU Dongmei;ZHAO Juanli;YUAN Jianqin(College of Life Sciences,Shanxi Agricultural University,Taigu,Shanxi 030801)
出处
《核农学报》
CAS
CSCD
北大核心
2021年第12期2821-2829,共9页
Journal of Nuclear Agricultural Sciences
基金
转基因生物新品种培育重大专项(2018ZX08012001-009)
关键词
转基因大豆
子代大鼠
脏器系数
显微结构
葡萄糖转运蛋白8
genetically modified soybean
rat
organ coefficient
microstructure
glucose transporter 8(GLUT8)
