摘要
目的:研究白藜芦醇(Resveratrol,Res)对脓毒症大鼠急性肺损伤(Acute lung injury,ALI)的保护作用及对ALI大鼠肺组织中高迁移率族蛋白(high mobility group box 1 protein,HMGBl)及Toll样受体4(Toll-like receptor 4,TLR4)mRNA表达的影响。方法:将大鼠随机分为假手术组(Sham组)、脓毒症组(Sep组)和白藜芦醇组(Res组),采用盲肠结扎穿刺术(Cecal ligation and puncture,CLP)制备脓毒症相关性ALI大鼠模型,造模1 h后,Res组给予40mg/kg,Sham和Sep组给予等量生理盐水,均经股静脉注射。各组分别于2、6、12和24h,取大鼠肺组织,HE染色观察病理形态学变化,RT-PCR检测脓毒症大鼠各器官组织HMGB1及TLR4的mRNA的动态表达变化。结果:Res组在造模后12 h,肺组织病理变化与Sep组相比有所减轻,24 h Res作用达到最强,各种病理改变明显减轻,部分肺组织开始接近正常形态;Res组与Sepsis组比较,肺组织HGMB1及TLR4 mRNA表达明显下调。结论:Res可以通过下调肺组织HGMB1及TLR4 mRNA表达减轻脓毒症诱导的肺损伤。
Objective: To investigate the protective effect of resveratrol on rats with acute lung injury(ALI) induced by sepsis and its influence on mRNA expressions of high mobility group box 1 protein(HMGBl) and Toll-like receptor 4(TLR4) in the injured lung tissue.Methods:Wistar rats were randomly divided into sham operation(Sham),sepsis(Sep) and resveratrol(Res) groups.Sepsis-induced ALI model was established by cecal ligation and puncture(CLP),and then,1 h later,the rats in Res group were injected i.v.with resveratrol at a dosage of40 mg/kg,while those in Sham and Sep groups with physiological saline each at an equal volume.The lung tissues of rats in various groups were collected 2,6,12 and 24 h after successful modeling,and observed for morphological change by HE staining and messenger RNA(mRNA) expression levels of HMGBl and TLR4 in lung tissue by Reverse transcriptase PCR(RT-PCR).Results: The pathological change of lung tissues of rats in Res group 12 h after modeling was relieved as compared with those in Sep group,while the morphology of partial lung tissue was nearly normal 24 h after modeling(P<0.05).Conclusion Res can alleviate the lung injury induced by sepsis by down-regulating the mRNA expression of HGMB1 and TLR4 in the lung tissue.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2015年第2期138-139,共2页
Pharmacology and Clinics of Chinese Materia Medica
