摘要
目的:探讨脓毒血症过程中大鼠脑组织Na^+、K^+、Ca^(2+)含量变化及机制,了解尼莫通的脑保护作用,为进一步研究与治疗脓毒血症提供试验依据。方法:将150只Wistar大鼠随机分为对照组、脓毒血症组、尼莫通治疗组,每组50只。脓毒血症组行盲肠结扎穿刺术(cecal ligation and puncture,CLP);治疗组行CLP术,并在腹腔注射尼莫通;对照组行假手术。每组按术后不同时间分为4、8、12、16、20 h组,每组10只。测定各组大鼠脑组织Na^+、K^+、Ca^(2+)含量及脑组织含水量。结果:脓毒血症20 h组脑组织Na^+、Ca^(2+)含量及含水量增加,K^+降低,与脓毒血症组其它时点比较差异均有统计学意义(P<0.05)。脓毒血症20 h组分别与对照组、治疗组20 h比较,Na^+、Ca^(2+)含量及脑组织含水量明显增加,差异有统计学意义(P<0.05)。结论:脓毒血症晚期脑组织Na^+、Ca^(2+)含量及含水量增加,出现脑水肿,脓毒血症时脑组织明显的器质性改变出现较晚。尼莫通对脓毒血症脑组织的病理性改变有一定的预防与治疗作用。
Objective： To explore the mechanism of ehange of content of Na^＋ , K^＋ , Ca^2＋ in brain in septic rat, to know about the protective function of Nimotop. Methods： 150 Wistar rats were randomly devided into control group, sepsis group and Nimotop treated group （n =50 each）. The sepsis model was made by cecal ligation and puncture （CLP） in the sepsis group. Without CLP, the control group got the same treatment. Nimotop treated group received CLP and Nimoltop by peritoneal injection. And each group was also divided into 5 groups of 4, 8, 12, 16, 20 h after operation （n =10 each）. Following indexes were measured in each group： content of Na^＋ , K^＋ , Ca^2＋ and water of brain tissue. Results： Compared with other time in the sepsis group, the content of Na^＋ , Ca^2＋ and water of rats, brain tissues at 20 h increased significantly（P〈0.05）. While the content of K^＋ decreased（P〈0.05）. The content of Na^＋ ,Ca^2＋ and water at 20 h in the sepsis group increased significantly compared with 20 h in the control group and in Nimotop treated group （P 〈0.05）. Conclusion： Content of Na^＋ Ca^＋ and water of brain tissue increases and brain edema appears in the advanced stage of sepsis. The appearnce of brain＇s parenchymatous change maybe comes later during sepsis. Nimotop has the preventive and therapeutical effects for the pathological change of brain tissue during sepsis. The experiment provide the basis for the study Of sepsis in preclinic and in clinic.
出处
《新疆医科大学学报》
CAS
2008年第3期277-279,共3页
Journal of Xinjiang Medical University
