摘要
目的探讨氯胺酮对内毒素诱导的肺动脉和体动脉环张力的影响,为临床指导感染性休克患者的治疗提供依据。方法将兔离体血管环(16个体动脉血管环,15个肺动脉血管环)随机分为4组:正常体动脉血管环组(SA组,n=8),正常肺动脉血管环组(PA组,n=8),内毒素预孵育的体动脉血管环组(LSA组,n=8),内毒素预孵育的肺动脉血管环组(LPA组,n=7)。首先,测量4组血管环对苯肾及乙酰胆碱(ACH)的反应值;然后,将血管环冲洗到平衡张力;再加入氯胺酮预孵育30 min后,测量其对苯肾及ACH的反应值。结果与SA组比较,LPA组减弱了ACH 1μm和10μm的舒张反应(P<0.05),LSA组对其收缩及舒张反应无影响。氯胺酮预孵育后,SA组氯胺酮抑制了ACH 10μm和100μm的舒张反应(P<0.05),但对PE的收缩无影响。在PA组,氯胺酮对PE的收缩反应及ACH的舒张反应(1μm、10μm、100μm)均无影响。氯胺酮预孵育后,在LSA组,氯胺酮增强了PE的收缩反应(P<0.05),降低了ACH 100μm的舒张反应(P<0.01)。在LPA组,氯胺酮降低了ACH 100μm的舒张反应(P<0.05)。LPA组氯胺酮对ACH 1μm、10μm和100μm的舒张反应弱于PA组(P<0.05)。SA及LSA组相比较,血管的收缩及舒张反应差异无统计学意义(P>0.05)。LSA组与LPA组比较,氯胺酮对PE及ACH 1μm和10μm的影响差异无统计学意义(P>0.05);但对ACH 100μm的舒张反应LPA组弱于LSA组(P<0.05)。结论氯胺酮降低了正常体动脉的舒张反应,而对正常肺动脉无显著影响;脂多糖(LPS)孵育后,氯胺酮增强了体动脉的收缩反应,同时,降低了体动脉及肺动脉的舒张反应。
Objective To investigate regulatory roles of ketamine on isolated rabbit pulmonary and systemic arteries during septic shock, to provide basis for clinical treatment of patients. Methods The blood vessels （ 16 individual arterial rings, 15 pulmonary arterial rings ）of the rabbit was randomly divided into 4 groups：the normal artery ring group （SA group, n=8 ） , normal pulmonary artery ring group（ PA group,n = 8 ）, systemic arterial endotoxin preincubation ring group （ LSA group, n = 8 ） and pulmonary vascular endotoxin preincubation ring group（ LPA group, n=7 ）. First of all, to measure the response value of 4 groups of vascular rings to the kidney and acetylcholine （ACH） ;then to wash the blood vessels to the equilibrium tension;to measure the response value of benzene and ACH by adding ketamine to incubate for 30 minutes. Results Compared with SA group,LPA group reduced the relaxation response with ACH 1μm and 10 μm（ P 〈0. 05 ） , LSA group had no effect on the contraction and relaxation response. After ketamine pretreatment, ACH 10μm 和 100 μm were inhibited by ketamine in SA group（P 〈0. 05） ,while it had no effect on the contraction of PE. In PA group, ketamine had no effect on the contractile response of PE and the relaxation response of ACH （1μm, 10 μm, 100μm）. After ketamine pretreatment ,in LSA group, ketamine increased the contractile response of PE（ P 〈 0. 05 ） ,and reduced the ACH 100 p.m diastolic response（ P 〈 0. 01 ）. In LPA group, ketamine reduced the ACH 100 μm diastolic response （ P 〈 0. 05 ）. In LPA group, ketamine relaxation response on ACH 1μm, 10μm and 100 μm was weaker than that in PA group （P 〈 0. 05 ）. Compared with SA group and LSA group, there was no statistically significant difference in blood vessel contraction and relaxation （P 〉 0. 05 ）. Compared with LSA group,the effect of ketamine on PE and ACH 1μm and 10 μm was not statistically significant （P 〉 0. 05 ） ;while the diastolic response of ACH
出处
《临床军医杂志》
CAS
2017年第2期180-183,共4页
Clinical Journal of Medical Officers
基金
沈阳市重点应用基础课题(071021)
沈阳市重点应用基础课题(F14-231-60)
关键词
体动脉
肺动脉
氯胺酮
内毒素
Systemic artery
Pulmonary artery
Ketamine
Endotoxin
