摘要
目的:观察氯胺酮(KT)对感染性休克大鼠一般情况、肝脏病理组织学、肺组织干湿重比、血流动力学及20 h死亡率的影响。方法:取健康成年雄性SD大鼠89只,采用盲肠结扎加穿孔(CLP)法复制感染性休克模型。随机分为4组:假CLP组、CLP组、KTⅠ组和KTⅡ组。假CLP和CLP组术前30 min经股静脉持续输注生理盐水5 ml/(kg.h),KTⅠ和KTⅡ组分别输注氯胺酮5 mg/(kg.h)和10 mg/(kg.h)。取新鲜肝组织进行石蜡切片,苏木素-伊红(HE)染色;微波炉烘干微量天平称肺干湿重,计算比值;股动脉穿刺置管监测平均动脉压(MAP),心率(HR)。结果:CLP组术后大鼠一般情况较差;肉眼观各脏器充血,发黑;肝脏病理组织学观察门管区有较多量的炎性细胞浸润;肺干湿重比各组间比较差异不显著(P>0.05);MAP进行性下降,HR则先加快后减慢,各组间比较差异显著(P<0.05)。结论:氯胺酮对感染性休克大鼠具有保护效应。
Objective： To investigate the effect of ketamine on the histopathologic changes of liver and dry/wet weight ratio of lung tissue and hemodynamics in septic shock rats and the mortality during 20 hours after operation. Methods. Eighty-nine healthy male Sprague-Dawlay （SD） rats weighing （225 ± 25） g were divided into four groups： the sham CLP group, CLP group, ketamine Ⅰ （KT Ⅰ ） and ketamine Ⅱ （KTⅡ） group. The rats were subjected to cecal ligation and puncture （CLP, a model of septic shock）. Thirty mintues before CLP, saline （0.9%） was infused continously at a rate of 5 ml/（kg· h） through the left femoral vein cannula in the sham CLP and CLP group. Ketamine （5 ml/（kg· h） and 10 ml/（kg · h）, respectively） was infused continously in KT Ⅰand KT Ⅱgroup. The right femoral artey was cannulated to monitor mean arterial pressure and heart rates. Results. CLP produced progressive hypotension, and the hemodynamic responses were attenuated（P〈0.05） in KT Ⅰ and KT Ⅱ group. Mortality of 20 hours in KT Ⅰ group was the lowest. Conclusion： Ketamine administration has protective effect against septic shock in rats.
出处
《武汉大学学报（医学版）》
CAS
2006年第1期87-90,i0002,共5页
Medical Journal of Wuhan University
基金
湖北省自然科学基金资助课题(No.2002AB147)
