摘要
目的:探究当归多糖保护大鼠脑缺血再灌注损伤的机制。方法:体外实验使用LPS刺激小鼠海马神经元细胞HT22,MMT法检测细胞存活率、Western Blotting法检测细胞凋亡蛋白水平。体内实验中60只大鼠随机分为对照组、假手术组、模型组和低中高3个APS给药组,每组10只,3个APS给药组分别灌胃50、100和200 mg/kg APS,其余3组灌胃相应体积的溶剂,每天1次,预防1周后使用改良后的线栓法构建大鼠脑缺血模型,对照组不处理,假手术组进行同样的手术操作但不结扎颈总动脉和颈外动脉,脑缺血2 h后恢复供血,继续治疗给药一周后处理动物。结果:不同浓度APS能够有效的促进HT22细胞存活(P<0.05),同时3个APS给药组能够在不同程度上调Bcl-2蛋白,显著下调Bax和Cyto C蛋白水平。造模后模型组大鼠体质量呈现快速下降,3个APS给药组大鼠体质量在造模后前3 d体质量出现下降,后期平缓上升。在模型后期模型组和3个APS给药组大鼠分别出现了50%、25%、12.5%及12.5%的死亡率。模型组大鼠脑组织梗死体积比达到29.73%,其极显著高于正常对照组(P=0.000),APS组大鼠脑组织梗死体积较模型组显著下降(P=0.000),APS组大鼠脑海马组织中Bcl-2蛋白水平较模型组有所上调,同时Bax和Cyto C蛋白水平显著下降,APS组中VEGF和CD31的基因表达和蛋白水平均出现了极强的表达(P<0.001)。结论:低分子量的当归多糖可能通过抑制海马神经元细胞的凋亡和促进脑组织血管生成进而缓解大鼠脑缺血再灌注损伤。
Objective:To investigate the mechanism of Angelica sinensis polysaccharide protecting cerebral ischemia-reperfusion injury in rats.Methods:In vitro,LPS was used to stimulate mice hippocampal neuronal cell line HT22.Cell viability was detected by MMT assay and apoptosis protein was detected by Western blotting.In vivo,60 rats were randomly divided into control group,sham operation group,model group and low-to-medium-high APS administration groups,10 rats in each group,and three APS administration groups were intragastrically administered 50,100 and 200 mg/kg APSrespectively.The other three groups were intragastrically administered with the corresponding volume of solvent once a day.After one weekprevention,the rat model of cerebral ischemia was constructed using the modified suture method.The control group was not treated.The sham operation group performed the same operation but did not ligature the neck.The common arteries and external carotid arteries were returned to the blood supply 2 hours after cerebral ischemia,and the animals were treated one week after treatment.Results:Different concentrations of APS could effectively promote the survival of HT22 cells(P<0.05).At the same time,the three APS-administered groups could regulate Bcl-2 protein to different degrees and significantly down-regulate the levels of Bax and Cyto C protein.After modeling,the body weight of the model group showed a rapid decline.The body weight of the three APS-administered groups decreased in the first 3 days after modeling,and gradually increased in the later stage.Mortality of 50%,25%,12.5%,and 12.5%occurred in the model late model group and the three APS-administered groups,respectively.The infarct volume ratio of the brain tissue of the model group reached 29.73%,which was significantly higher than that of the normal control group(P=0.000).The infarct volume of the brain tissue of the APS group was significantly lower than that of the model group(P=0.000).The APS group’s level of Bcl-2 protein in hippocampus was up-regulated co
作者
李晶晶
雷涛
林俊
廖维靖
LI Jingjing;LEI Tao;LIN Jun;LIAO Weijing(Department of Pediatric Rehabilitation,Wuhan Children’s Hospital(Wuhan Maternal and Child Healthcare Hospital),Tongji Medical College,Huazhong University of Science&Technology,Wuhan 430000,Hubei,China;Department of Rehabilitation Medicine,Zhongnan Hospital Affiliated to Wuhan University,Wuhan 430000,Hubei,China)
出处
《中华中医药学刊》
CAS
北大核心
2019年第9期2272-2276,2317,共6页
Chinese Archives of Traditional Chinese Medicine
基金
湖北省科技计划项目(2017CFC825)
关键词
当归多糖
缺血再灌注
血管生成
细胞凋亡
Angelica polysaccharide
ischemia-reperfusion
angiogenesis
apoptosis
