摘要
茶轮斑病是茶树的一种重要叶部病害,防治该病害通常使用化学杀菌剂。化学杀菌剂的频繁使用易使病菌产生抗药性并造成农药残留,因此,需要更为安全有效的防治方法。诺沃霉素A是由植生链霉菌HEBRC-20821分泌的一种新型32元环大环内酯类化合物。本研究探索了诺沃霉素A在离体条件下对拟盘多毛孢的抑菌活性及对感染茶轮斑病离体叶片的防治效果。诺沃霉素A在离体条件下对茶拟盘多毛孢的菌丝生长具有强烈抑制作用,抑制中浓度为2.11μg/mL。当用5μg/mL诺沃霉素A处理菌丝后,在显微镜下可见茶拟盘多毛孢菌丝形态发生异常。在离体叶片条件下,诺沃霉素A在75和150μg/mL浓度下对茶轮斑病防效分别为68.29%和100%。在2 000和4 000μg/mL浓度下诺沃霉素A对茶叶没有产生药害。诺沃霉素A有望成为防治茶轮斑病的一种新型药剂。
Gray blight is an important foliar disease of tea plant. Controlling this disease usually relies on chemical fungicides, but overusing of fungicide may lead to resistance of pathogen and pesticide residue, so it is necessary to search for alternative methods. Novonestmycin A is a new 32-membered macrolide compounds produced by Streptomyces phytohabitans HBERC-20821. In order to explore the application of novonestmycin A on controlling gray blight of tea, the bioassay was conducted in vitro and in detached leaves. It strongly inhibited mycelial growth of Pestalotiopsis theae in vitro, with the median inhibitory concentration of 2.11 μg/mL. When treated with novonestmycin A at the concentration of 5 μg/mL, hyphae morphology became irregular in optical microscope. When the leaves were sprayed with novonestmycin A at the concentration of 75 and 150 μg/mL,the control efficiencies were 68.29% and 100%, respectively. Novonestmycin A had no injure on epidermis of tea at the concentration of 2 000 and 4 000 μg/mL, thus it has potential as a new fungicide in controlling gray blight of tea.
作者
黄大野
万中义
朱志刚
姚经武
杨妮娜
曹春霞
张亚妮
刘晓艳
饶犇
HUANG Daye;WAN Zhongyi;ZHU Zhigang;YAO Jingwu;YANG Nina;CAO Chunxia;ZHANG Yani;LIU Xiaoyan;RAO Ben(Hubei Biopesticide Engineering Research Center,Wuhan 430064,China;Institute of Plant Protection and Soil Fertilizer,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)
出处
《植物保护》
CAS
CSCD
北大核心
2019年第2期243-246,共4页
Plant Protection
基金
国家重点研发计划(2016YFD020090501)
湖北省技术创新专项(2017ABA160)
湖北省农业科技创新中心创新团队项目(2016-620-000-001-038)
关键词
茶轮斑病
大环内酯化合物
诺沃霉素A
防效
gray blight of tea
macrolide compounds
novonestmycin A
control effect
