摘要
[目的]探讨创伤性股骨头坏死(TONFH)的血清蛋白质组学差异质,寻找并鉴定TONFH的潜在血清生物学标志物。[方法]分别采集TONFH患者(实验组)、正常体检者(对照组)血清各5例,采用TMT联合2DLC-MS/MS技术进行蛋白组学分析,寻找并鉴定差异表达的蛋白质。[结果]质谱共筛选出有意义差异蛋白344个,并鉴定出15种显著性差异蛋白(P<0.05),其中与正常组对比,实验组中抗粘蛋白1轻链可变区、补体C5(C5)、冷凝集蛋白FS-2 H链、纤维连接蛋白(FN1)、妊娠区带蛋白(PZP)、β肌动蛋白(ACTB)、甘露糖结合蛋白C(MBL2)等7种蛋白表达上调,Igλ链V区4A、多巴胺β-羟化酶(DBH)、免疫球蛋白重链可变区、CD5抗原(CD5L)、免疫球蛋白J链(JCHAIN)、补充C1r子组件(C1R)、抗凝血酶III (Antithrombin-III)、凝溶胶蛋白(GSN)等8种蛋白表达下调。[结论]采用同位素相对标记与绝对定量技术(TMT)联合二维液相色谱-串联质谱(2D-LC-MS/MS),成功筛选出了TONFH的血清差异表达蛋白质,为寻找TONFH特异性标志物及进一步阐明TONFH的发病机制提供了依据。
[Objective] To investigate the serum proteomics of traumatic osteonecrosis of the femoral head(TONFH) and identify potential serum biomarkers of TONFH. [Methods] Serum samples were obtained from 5 patients with TONFH and 5 normal persons. The tandem mass tags(TMT) combined with 2 D-LC-MS/MS techniques were used for proteomic analysis to find and identify differentially expressed proteins. [Results] A total of 344 genes with significant differences were screened out by mass spectrometry, and 15 proteins with significant differences were identified(P<0.05). Compared with the normal group, 7 proteins proved up-regulated in the TONFH group, including the anti-viscoprotein 1 light chain variable region, complement C5(C5), condensing protein FS-2 H chain, fibronectin(FN1), pregnancy zone band protein(PZP), β actin(ACTB), and mannose-binding protein C(MBL2). Conversely, 8 proteins was down-regulated in the TONFH group, such as Igλ chain V region4 A, dopamine β-hydroxylase(DBH), immunoglobulin heavy chain variable region, CD5 antigen(CD5 L), immunoglobulin J chain(JCHAIN), complementary C1 r subcomponent(C1 R), and antithrombin-III(Gelsolin, GSN). [Conclusion] The differentially expressed proteins of TONFH were successfully screened by isotope relative labeling and absolute quantification(TMT)combined with two-dimensional liquid chromatography-tandem mass spectrometry(2 D-LC-MS/MS) for the purpose of searching for TONFH specific markers, which should provide the basis for further elucidation of the pathogenesis of TONFH.
作者
曾平
陈金龙
李金溢
秦刚
何凯毅
杜敏东
孙斌
李德剑
周怡
ZENG Ping;CHEN Jin-long;LI Jin-yi;QIN Gang;HE Kai-yi;DU Min-dong;SUN Bin;LI De-jian;ZHOU Yi(First Affiliated Hospital,Guangxi University of Traditional Chinese Medicine,Nanning 530023,China;Guangxi University of Traditional Chinese Medicine,Nanning 530001,China;Medical Science Experiment Center,Guangxi Medical University,Nanning 530021,China)
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2019年第5期453-458,共6页
Orthopedic Journal of China
基金
国家自然科学基金项目(编号:81360551)
国家中医药管理局项目(编号:JDZX2015272)
广西壮族自治区中医药管理局科技项目(编号:GZMZ1206)
广西壮族自治区医疗卫生重点科研项目(编号:2011053)
关键词
创伤性股骨头坏死
蛋白质组学
TMT技术
血清
traumatic osteonecrosis of the femoral head(TONFH)
proteomics
TMT technology
serum
