摘要
采用大孔吸附树脂柱色谱、硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及半制备型高效液相色谱等多种色谱分离技术从鄂西香茶菜干燥地上部分水提液中分离得到10个化合物,综合运用UV,IR,MS,NMR等光波谱学技术鉴定了10个化合物的结构,分别为毛叶酯(1),毛栲利素(2),毛叶香菜醇(3),四川香茶菜丙素(4),毛叶香菜素(5),河北冬凌草素(k)(6),冬凌草丙素(7),延命草醇(8),冬凌草甲素(9),延命草醇-1-β-葡萄糖苷(10),其中化合物1～8和10均为首次从鄂西香茶菜中分离得到。活性测试结果表明,化合物2和9对4种肿瘤细胞均有较强的抑制作用,IC50为2. 25～9. 32μmol·L-1。
The chemical constituents of the water extraction of the aerial parts of Isodon henryi were investigated by various chromatographic methods including D-101 macroporous adsorptive resins,silica gel,sephadex LH-20,and semi-preparative HPLC. As a result,ten compounds were separated and purified. By analyses of the UV,IR,MS,NMR spectra,their structures were determined as rabdosinate( 1),lasiokaurin( 2),epinodosinol( 3),rabdosichuanin C( 4),epinodosin( 5),hebeirubescensin k( 6),rubescensin C( 7),enmenol( 8),oridonin( 9),and enmenol-1-β-glucoside( 10). Compounds 1-8 and 10 were isolated from I. henryi for the first time. Compounds 2 and 9 showed inhibitory effects against four tumor cells,with IC50 values of 2. 25-9. 32 μmol·L-1.
作者
张玲霞
刘雅琳
吴鸿
陈随清
代丽萍
ZHANG Ling-xia;LIU Ya-lin;WU Hong;CHEN Sui-qing;DAI Li-ping(College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Laboratory of Cell Imaging,Henan University of Chinese Medicine,Zhengzhou 450002,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第2期319-323,共5页
China Journal of Chinese Materia Medica
基金
河南省自然科学基金项目(162300410188
162300410187)
河南省高校基本业务经费(2014KYYWF-QN02)
关键词
鄂西香茶菜
结构鉴定
二萜
细胞毒
Isodon henryi
structural identification
diterpenes
cytotoxic
