摘要
采用超声波-微波协同提取黑果枸杞叶醇提物,利用大孔吸附树脂分离富集黑果枸杞叶总黄酮,采用Sephadex LH-20,分离纯化槲皮素。利用高效液相色谱-质谱联用法(high performance liquid chromatography and mass spectrometry,HPLC-MS)对所得的槲皮素进行结构鉴定。通过考察槲皮素的总抗氧化活性、清除DPPH自由基、ABTS+自由基、超氧阴离子自由基以及脂质过氧化体系中脂质过氧化抑制率5个指标,由此评价槲皮素的体外抗氧化活性。研究结果表明,槲皮素在5种自由基中清除ABTS+自由基能力最强,浓度在300 nmol/L～400 nmol/L时清除率达68.0%。清除DPPH自由基能力和清除超氧阴离子自由基能力基本持平。
The total flavonoids of Leaves of Lycium ruthenicum Murr.were separated and enriched by macroporous adsorption resins,and the quercetin was separated and purified by Sephadex LH-20.The structure of the quercetin was identified by high performance liquid chromatography and mass spectrometry(HPLC-MS).The antioxidant activity of the quercetin in vitro was evaluated by five factors:determination of total antioxidant capacity,scavenging effects of DPPH free radical,ABTS+free radical and super-oxide anion,and lipid peroxidation inhibition rate in lipid peroxide system.The results showed that the quercetin had the strongest ability to remove ABTS+free radical.Under the concentration of quercetin in 300 nmol/L-400 nmol/L,the clearance rate can reach 68.0%.The quercetin had the almost same ability to clear DPPH free radical and super-oxide anion free radical.
作者
段亚云
李建颖
程瑶
白金金
DUAN Ya-yun;LI Jian-ying;CHENG Yao;BAI Jin-jin(College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin Key Laboratory of Food Biotechnology,Tianjin 300134,China)
出处
《食品研究与开发》
CAS
北大核心
2019年第5期59-64,共6页
Food Research and Development
基金
天津市大学生创新训练计划项目(201810069053)
关键词
黑果枸杞叶
槲皮素
制备
高效液相色谱-质谱联用法
抗氧化
leaves of Lycium ruthenicum Murr.
quercetin
preparation
high performance liquid chromatography-tandem mass spectrometry(HPLC-MS)
antioxidant
