摘要
目的:探讨姜黄素对过度训练所致大鼠氧化应激、肾脏细胞凋亡的作用及其机制。方法:7周龄SPF级雄性Wistar大鼠分为对照组(C组,n=12)、过度训练组(OM组,n=11)、姜黄素+过度训练组(COM组,n=14)。C组不进行任何运动干预,OM组、COM组大鼠进行8周递增负荷游泳训练。训练期间,COM组以200 mg/(kg·d)、5 ml/kg姜黄素进行灌胃,其他组灌胃等体积0.5%浓度羧甲基纤维素纳。末次训练后24 h,光镜观察肾脏组织病理学改变,取血液、肾脏组织检测相关生化指标。结果:8周递增负荷游泳训练后,光镜下C组大鼠肾脏组织结构正常; OM组出现组织病理学改变; COM组较OM组减轻。与C组比较,OM组血清皮质酮(Cor)、肌酐(Cr)和尿素氮(BUN)水平均升高(P<0.01),睾酮(T)水平降低(P<0.01);肾脏核因子E2相关因子2(Nrf2)表达水平无显著变化(P>0.05),血红素氧合酶1(HO-1)表达水平降低(P<0.05),总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD)活性降低(P<0.01),丙二醛(MDA)浓度升高(P<0.01);肾脏细胞凋亡水平升高(P<0.01),肾脏抗凋亡蛋白B淋巴细胞瘤因子-2(Bcl-2)表达减弱(P<0.01),促凋亡蛋白Bcl-2相关X蛋白(Bax)表达增强(P<0.01)。与OM组比较,COM组血清Cor水平(P<0.01)降低,T水平升高(P<0.01),Cr和BUN水平均降低(P<0.05);肾脏Nrf2和HO-1表达增强(P<0.05),T-AOC和SOD活性升高(P<0.01),MDA浓度降低(P<0.05);肾脏细胞凋亡水平降低(P<0.05),Bcl-2表达增强(P<0.05),Bax表达减弱(P<0.01)。组间T/Cor比值变化趋势与T变化相一致,Bcl-2/Bax比值变化趋势与Bcl-2变化相一致。结论:8周递增负荷游泳训练引发大鼠过度训练,氧化应激加剧并加速肾脏细胞凋亡,肾脏组织发生病理改变及功能异常。姜黄素通过上调Nrf2、HO-1蛋白表达,有效缓解过度训练引发的氧化应激,从而增强Bcl-2表达,减弱Bax表达,抑制大鼠肾脏细胞凋亡,保护肾脏组织结构和功能正常。
Objective:To study the effects and mechanisms of curcumin alleviating oxidative stress induced by overtraining and inhibiting renal apoptosis in rats.Methods:Male Wistar rats of 7 weeks old were divided into control group(C group,12),overtraining group(OM group,11),curcumin+overtraining group(COM group,14).Group C did not undergo any exercise intervention.Rats in OM group and COM group underwent 8-week incremental load swimming training.During the training,the COM group was treated with curcumin at the dose of 200 mg/(kg·d)in the volume as 5 ml/kg by intragastric administration,and the other groups was treated with an equal volume of 0.5%carboxymethylcellulose.Twenty-four hours after the last training,renal histopathological changes were observed by light microscopy,related biochemical indicators in blood and renal tissue were detected.Results:The results showed that after 8 weeks of incremental load swimming training,the renal tissue structure of group C was normal under light microscope;histopathological changes were observed in OM group;COM group was significantly relieved compared with OM group.Compared with group C,serum levels of corticosterone(Cor),creatinine(Cr)and blood urea nitrogen(BUN)in OM group were increased(P<0.01),serum level of testosterone(T)was lower(P<0.01);the expression of nuclear factor erythroid 2-related factor 2(Nrf2)was not changed significantly(P>0.05),while the expression of heme oxygenase-1(HO-1)was decreased(P<0.05),total antioxidant capacity(T-AOC)and superoxide dismutase(SOD)activity were decreased(P<0.01),malondialdehyde(MDA)concentration was increased(P<0.01);the renal apoptosis was increased(P<0.01),the expression of anti-apoptotic B cell lymphoma-2 protein(Bcl-2)was decreased(P<0.01),and the expression of proapoptotic Bcl-2 associated X protein(Bax)was increased(P<0.01).Compared with the OM group,Cor level was decreased(P<0.01)in the COM group,T level was increased(P<0.01),Cr and BUN levels were lower(P<0.05);the expression of Nrf2 and HO-1 were increased(P<0.05),T-AOC and S
作者
胡戈
曹卉
周海涛
曹建民
郭娴
牛衍龙
包欣悦
任奕
李倩
张涛
赵佳晖
HU Ge;CAO Hui;ZHOU Hai-tao;CAO Jian-min;GUO Xian;NIU Yan-long;BAO Xin-yue;REN Yi;LI Qian;ZHANG Tao;ZHAO Jia-hui(Beijing Sport University,Beijing 100084;Beijing Union University,Beijing 100023;Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University,Beijing 100191;Gannan Medical University,Ganzhou 341000,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2018年第6期513-518,583-584,共7页
Chinese Journal of Applied Physiology
基金
北京市朝阳区协同创新项目(CYXC1508)
北京市高等学校高水平人才交叉培养"实培计划"项目
北京联合大学2018年"启明星"大学生科技创新创业项目(201811417025
201811417SJ088)
中央高校基本科研业务费专项资金资助课题(2018PT014)
