摘要
目的研究楤木总皂苷对T2DM小鼠血糖及肾功能的影响,并从抗氧化及细胞凋亡角度探讨其作用机制。方法将90只雄性小鼠按随机数字表法分为正常组、模型组、阳性对照组及楤木总皂苷低、中、高剂量组。除正常组外,其余各组小鼠采用高糖高脂饲料1个月及一次性大剂量注射STZ诱导T2DM小鼠模型。正常组和模型组灌胃等体积生理盐水,阳性对照组灌胃贝那普利溶液1 mg/kg,低、中和高剂量组灌胃楤木总皂苷30、60、120 mg/kg,按1 ml/kg小鼠体重灌胃,1次/d,连续灌胃6周后取材。检测小鼠血清胰岛素和SOD、MDA水平,检测小鼠尿液肌酐(creatinine, Cr)、尿素氮(urea nitrogen, UN)、尿酸(uric acid, UA)水平,采用免疫组化染色和Western blot检测肾组织中Bax和Bcl-2蛋白表达。结果与模型组比较,楤木总皂苷低、中和高剂量组小鼠血糖和胰岛素抵抗指数均明显降低(P<0.05);小鼠尿液UN、Cr和UA水平降低(P<0.05);血清SOD升高,MDA水平降低(P<0.05);肾组织Bcl-2平均灰度值[(92.26±11.36)、(107.17±9.26)、(132.65±8.45)比(56.42±16.24)]升高,Bax平均灰度值[(152.62±9.86)、(124.48±10.36)、(92.29±10.10)比(171.38±15.18)]降低(P<0.05);Bax蛋白[(0.81±0.06)、(0.75±0.07)、(0.52±0.09)比(2.02±0.09)]表达降低、Bcl-2蛋白[(0.92±0.08)、(0.94±0.12)、(1.27±0.07)比(0.30±0.09)]表达升高(P<0.05)。结论楤木总皂苷可降低T2DM小鼠血糖水平,改善肾功能,其作用机制可能与提高抗氧化能力、抑制肾脏细胞凋亡有关。
Objective To study the effect of aralia total saponins on renal function of type 2 diabetic mice, and its effect on the Bax and Bcl-2 protein in renal tissues, in order to provide some references for the development of aralia total saponins. Methods The mice were divided into the normal group, model group, positive control group, low, medium and high dose aralia total saponins group by random number method. Except the normal group, the others were received with high-fat diet for one month+one-time large dose of streptozotocin (STZ) to induce type 2 diabetic model, and then the mice in the normal group and the model group were intragastrically administered with the same volume of normal saline, and the mice in the positive control group was given 1 mg/kg of benazepril solution, and the low, medium and high dose groups were given 30, 60, 120 mg/kg aralia total saponins. The body weight of 1 ml/kg mice was intragastrically administered once a day. After treatment for 6 weeks they were sacrificed, and the serum insulin, and SOD and MDA levels were measured, the urine creatinine (Cr), urea nitrogen (UN), and uric acid (UA) levels were also measured. The immunohistochemistry and Western blot were used to detect the Bax and Bcl-2 protein expression in kidney tissues. Results Compared with the model group, the blood glucose and insulin resistance index in the low, medium and high doses aralia total saponins group were significantly decreased (P<0.05);the levels of urine UN, Cr and UA significantly decreased (P<0.05);The serum SOD level increased and the MDA level significantly decreased (P<0.05). The average gray value of Bcl-2 increased (92.26 ± 11.36, 107.17 ± 9.26, 132.65 ± 8.45 vs. 56.42 ± 16.24) in kidney tissue. The average gray value of Bax (152.62 ± 9.86, 124.48 ± 10.36, 92.29 ± 10.10 vs. 171.38 ± 15.18) significantly decreased (P<0.05);Bax protein (0.81 ± 0.06, 0.75 ± 0.07, 0.52 ± 0.09 vs. 2.02 ± 0.09) significantly decreased, but Bcl-2 protein (0.92 ± 0.08, 0.94 ± 0.12, 1.27 ± 0.07 vs. 0.30 ± 0.
作者
曾德望
李红艳
赵世莉
杨椹
张云芳
李文豪
田鲁
Zeng Dewang;Li Hongyan;Zhao Shili;Yang Zhen;Zhang Yunfang;Li Wenhao;Tian Lu(Department of Nephrology,Guangzhou Huadu District People's Hospital,Guangzhou 510800,China)
出处
《国际中医中药杂志》
2019年第4期381-386,共6页
International Journal of Traditional Chinese Medicine
基金
广州市医学重点学科建设项目(2017-2019)
广东省科技计划项目(2014A020212519).
关键词
楤木
皂苷类
糖尿病
2型
肾脏
氧化性应激
细胞凋亡
小鼠
Aralia Chinensis
Saponins
Diabetes mellitus,type 2
Kidney
Oxidative stress
Apoptosis
Mice
