摘要
目的:探讨岭南头皮针通过自噬相关蛋白(Beclin-1、p-62、LC3B)-凋亡相关蛋白(Cleaved-Caspase3、Bcl-2)信号轴交互促进脑外伤昏迷大鼠苏醒机制。方法:将40只健康成年SD雄性大鼠按体重随机分为对照组、模型组、假针刺组、岭南头皮针组,每组各10只。采用自由落体撞击法构建外伤昏迷(TBI)大鼠模型,岭南头皮针组采用TBI+岭南头皮针法干预,假针刺组采用TBI+普通针刺法干预,比较各组大鼠神经功能缺损量表(mNSS)评分,HE染色观察皮层、海马组织病理变化,Western Blot法检测自噬蛋白[LC3B、p-62、Beclin-1]以及凋亡蛋白[Caspase-3、Bcl-2]水平的变化。结果:第0、7、14天,TBI组、假针刺组、岭南头皮针组神经功能缺损评分均高于对照组(P<0.01);第7、14天,假针刺组、岭南头皮针组神经功能缺损评分均低于TBI组(P<0.01),岭南头皮针组大鼠神经功能缺损评分均低于假针刺组(P<0.01),且第14天,岭南头皮针组大鼠神经功能缺损评分低于第7天神经功能缺损评分(P<0.01)。进行岭南头皮针刺治疗后,岭南头皮针组皮层神经元数量丰富,局部排列不规则,皮层可见少量神经元胞核皱缩深染,胞核、胞质分界不清晰,海马体神经细胞形态规则,排列整齐密集,胞核大而圆,呈均匀淡蓝色或蓝色,核仁清楚,细胞质丰富。与对照组相比,TBI组凋亡蛋白Cleaved-Caspase3表达升高(P<0.05),Bcl-2蛋白表达下降(P<0.05),自噬蛋白LC3B表达上升(P<0.05)、Beclin-1表达上升(P<0.01),p-62表达下降(P<0.05);与TBI组比,假针刺组凋亡蛋白Bcl-2蛋白表达升高(P<0.05),自噬蛋白LC3B表达下降(P<0.01),两组p-62、Beclin-1、Cleaved-Caspase3表达差异无统计学意义(P>0.05),岭南头皮针组,凋亡蛋白Bcl-2蛋白表达升高(P<0.01),自噬蛋白LC3B、Beclin-1表达下降(P<0.01),两组p-62、Cleaved-Caspase3表达差异无统计学意义(P>0.05)。结论:岭南头皮针可以有效促进颅脑损伤昏迷大鼠苏醒,其机制可能与
Objective:To investigate the mechanism of Lingnan scalp needle to promote the awakening of comatose rats with traumatic brain injury through the interaction of autophagy-related proteins(Beclin-1,p-62,LC3B)-apoptosis-related proteins(Cleaved-Caspase3,Bcl-2)signaling axis.Methods:Forty rats were randomly divided into control group,model group,sham needle group and Lingnan scalp needle group according to body weight,with 10 rats in each group.The rat model of traumatic coma(TBI)was constructed by free-fall impingement method,TBI+Lingnan scalp needle method was used in the Lingnan scalp needle group,TBI+ordinary acupuncture method was used in the sham needle group,the modified neurological severity score(mNSS)were compared in each group,the histopathological changes of cortex and hippocampus were observed by HE staining,and the autophagy proteins[LC3B,p-62,Beclin-1]and apoptotic proteins[Caspase-3,Bcl-2]were detected by Western blot method Level change.Results:On days 0,7 and 14,the mNSS of the TBI group,sham needle group and Lingnan scalp needle group were higher than those of the control group(P<0.01);on days 7 and 14,the mNSS of the sham needle group and Lingnan scalp needle group were lower than those of the TBI group(P<0.01),and the mNSS of the rats in the Lingnan scalp needle group were lower than those of the sham needle group(P<0.01),and on day 14,the mNSS of the rats in the Lingnan scalp needle group were lower than the mNSS on day 7(P<0.01).After Lingnan scalp needle treatment,the number of cortical neurons in the Lingnan scalp needle group was abundant,locally arranged irregularly,and a small number of neuronal nuclei were seen in the cortex wrinkled and deeply stained,with unclear demarcation of nucleus and cytoplasm,and the morphology of neuronal cells in the hippocampus was regular,arranged neatly and densely packed with a large,rounded nucleus that was uniformly light blue or blue in color,with a clear nucleolus and abundant cytoplasm.Compared with the control group,the expression of apoptotic protein
作者
杨东英
蔡伟彬
唐明
刘洋
秦敏
杨蕾
潘杰
YANG Dong-ying;CAI Wei-bin;TANG Ming;LIU Yang;QIN Min;YANG Lei;PAN Jie(Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510006;Guangdong Second Traditional Chinese Medicine Hospital(Guangdong Research Institute of Traditional Chinese Medicine Manuracturing Technology),Guangzhou,Guangdong 510095)
出处
《中医康复》
2024年第7期1-7,共7页
Traditional Chinese Medicine Rehabilitation
基金
广东省中医药管理局科研基金项目(No.20201029)。
关键词
岭南头皮针治疗
脑外伤
促醒
凋亡
自噬
Lingnan scalp needle
brain injury
promoting awakening
apoptosis
autophagy
