摘要
目的建立苦豆子提取物HPLC化学指纹图谱,测定苦豆子在不同提取物对小鼠的半数致死量(LD50),分析其'谱-毒'关系。方法采用75%乙醇回流法(ER)、水煎煮法(WD)、75%乙醇超声法(EU)和水超声法(WU)分别制备苦豆子提取物,并建立其HPLC指纹图谱,测定不同提取物的LD50;运用指纹图谱相似度评价系统软件分析不同提取方法下苦豆子提取物化学成分与LD50之间的关系。结果 ER、WD、EU、WU 4种提取方法制备的苦豆子提取物LD50分别为38.397、24.994、18.536、19.957g/kg;苦豆子提取物对小鼠脏器的眼观病变主要表现在肝、肾,其中以ER提取物毒性最大。苦豆子提取物HPLC 10个共有峰可分为2类,第4、10号峰及氧化苦参碱、氧化槐果碱与LD50呈显著负相关。结论构建了苦豆子'谱-毒'关系分析方法,其未鉴定出的第4、10号峰及氧化苦参碱和氧化槐果碱为毒性反应的主要化学成分。
Objective To establish the chemical fingerprint of Sophora alopecuroides extracts based on high performance liquid chromatography(HPLC),and determine the LD50 of different extracts of S.alopecuroides to analyze its'spectrum toxicity'relationship.Methods A series of extracts were prepared by 75%ethanol reflux(ER),water decoction(WD),75%ethanol ultrasound(EU)and water ultrasound(WU),and their fingerprints were established to determine the acute toxicity LD50 of different extracts.The relationship between chemical composition and acute toxicity LD50 of S.alopecuroides extracts were studied by means of fingerprint similarity evaluation system.Results The LD50 of ER,WD,EU,and WU extracts were 38.397,24.994,18.536,and 19.957 g/kg,respectively.The ocular lesions of mice viscera were mainly manifested in liver and kidney,and the toxicity of ER extracts was the greatest.The 10 common peaks of S.alopecuroides extracts can be divided into two categories;Peaks 4 and 10,oxymatrine and sophocarpidine were negatively correlated with acute toxicity LD50.Conclusion The spectral toxicity relationship analysis method of S.alopecuroides was constructed.The unidentified peaks 4,10 and oxymatrine and sophocarpidine were the main chemical components of the toxicity reaction,which laid a good foundation for clinical application and scientific and rational development of S.alopecuroides.
作者
刘学楠
贾娅倩
华永丽
张晓松
纪鹏
姚万玲
魏彦明
LIU Xue-nan;JIA Ya-qian;HUA Yong-li;ZHANG Xiao-song;JI Peng;YAO Wan-ling;WEI Yan-ming(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第11期2656-2664,共9页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(31560709)
国家自然科学基金资助项目(31472234)
国家肉牛/牦牛产业技术体系(CARS-37)
甘肃农业大学科技创新基金(青年导师扶持基金)(GAU-QDFC-2018-08)
