摘要
目的 观察表皮生长因子受体(EGFR)在增生性玻璃体视网膜病变(PVR)的增生膜(PRM)中的表达,并判断EGFR阳性细胞可能的来源。方法 PVR增生膜43例,按病程分为3期,采用免疫荧光法检测其EGFR表达并做定量分析;采用免疫荧光双重标记技术判定EGFR阳性细胞来源。结果 早期膜中阳性荧光总量约为中期膜的2.06倍,12例晚期膜均表达阴性;EGFR表达阳性的细胞,细胞角蛋白(cytokeratinpan)和巨噬细胞标记抗体(HAM-56)呈阳性表达。结论 视网膜色素上皮细胞及巨噬细胞具有EGFR,在PVR早期的发生发展起重要作用。
Objective To investigate the expression of epidermal growth factor receptor ( EGFR) in periretinal membrane (PRM) of proliferate vitreoretinopathy (PVR) and the identify of EGFR-positive cells in the membrane. Methods Fourty-three specimens from PRM of PVR were divided into three groups according to the course of PVR. The expression of EGFR and quantitative analysis were performed using immdnofluorescence staining. The EGFR-posilive cells was detected by immunofluorescence histochemcial double-staining. Results The total count of red fluorescence in the early stage of membrane was about 2. 06 times higher than that of in the middle stage of membrane. EGFR staining was negative in the late stage. The EGFR-positive cells showed staining positive for pancytokeratin and macrophage marker HAM-56. Conclusion Retinal pigment epithelial cells and macrophages express EGF, those cells play an important role in the development of the early stage of PVR.
出处
《眼科研究》
CSCD
北大核心
2003年第5期482-484,共3页
Chinese Ophthalmic Research
基金
国家自然科学资金(39970780)
