摘要
酸性条件下，少量铅（Ⅱ）对氧氟沙星的荧光强度有猝灭作用，据此建立了一种测定铅（Ⅱ）含量的荧光光谱分析法。在优化的实验条件下，其荧光猝灭强度与铅（Ⅱ）浓度在3．O×lO-7～1．O×lO-5mool·L-1范围内呈良好的线性关系，其检出限为8．4×10-8mol·L-1，相对标准偏差为3．2％（c=1．O×lO-6mol·L-1，n=11）。利用该法对三种蔬菜样品中铅（Ⅱ）含量的测定，回收率在94．46％～101．5％之间。
It was found that plumbum（Ⅱ） could quench the fluorescent intensity of ofioxacin in acid medium. Thus, a fluorescent spectral method for the determination of trace plumbum （Ⅱ） was developed. Under the optimal experiment conditions, the fluorescent quenching intensity responded linearly with plumbum （ Ⅱ ） concentration in the range of 3.O×lO-7～1.O×lO-5mool·L-1And a detection limit of 8.4×10-8mol·L-1 was estimated. The relative standard deviation （RSD） of this approach response to 1.0×l0-6mol· L-1 plumbum （11） was 3.2% for 11 successive measurements. This proposed method had been applied to detect the content of plumbum （ Ⅱ ） in three kinds of vegetable samples and the recovery was between 94.46% and 101.5%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第12期70-73,共4页
Science and Technology of Food Industry
基金
四川省广元市科技计划项目(GYKJ41429)
关键词
氧氟沙星
荧光猝灭法
铅(Ⅱ)
蔬菜
ofloxacin
fluorescence quenching method
plumbum（Ⅱ）
vegetable
