摘要
目的探讨长链非编码RNA(LncRNA)ZEB1-AS1表达水平与肝癌(HCC)患者临床预后的关系。方法采用RT-PCR法检测在西安市高陵区医院普通外科行手术切除或进行癌组织活检的67例HCC患者HCC组织和癌旁肝组织中LncRNAZEB1-AS1的表达水平,根据ZEB1-AS1在HCC组织中相对表达量将患者分为低表达组和高表达组,分析影响ZEB1-AS1表达水平的因素及其与生存率的关系。结果 HCC组织中ZEB1-AS1的表达水平高于癌旁肝组织(P<0.05);高表达组存在肝内转移、乙肝病史及术后复发的占比高于低表达组,Edmondson分级Ⅰ～Ⅱ级、TNM分期Ⅰ～Ⅱ期的占比低于低表达组(P<0.05)。高表达组术后生存率低于低表达组(P<0.05)。多因素分析结果显示,Edmondson分级、TNM分期、肝内转移、乙肝病史、术后复发及高表达ZEB1-AS1均是影响HCC患者临床预后的独立因素(P<0.05)。结论本研究证实ZEB1-AS1高表达与HCC恶性程度具有一定的相关性,其可作为预测HCC患者临床预后的独立危险因素及HCC潜在的治疗靶点。
Objective To investigate the relationship between the expression levels of ZEB1-AS1 of long non-coding RNA(LncRNA) and clinical prognosis of patients with hepatocellular carcinoma(HCC). Methods The expression levels of LncRNA-ZEB1-AS1 in HCC tissues and paracancerous liver tissue of 67 HCC patients who underwent surgical resection or biopsy of cancer tissue in general surgery department of the Hospital of Gaoling District were detected by RT-PCR.According to the relative expression levels of ZEB1-AS1 in HCC tissues, patients were divided into low expression group and high expression group, and the factors affecting the expression levels of ZEB1-AS1 and its relationship with survival rate were analyzed. Results The expression level of ZEB1-AS1 in HCC tissues was higher than that in paracancerous liver tissue(P<0.05).The proportions of intrahepatic metastasis, hepatitis B history and postoperative recurrence in the high expression group were higher than those in the low expression group, while the proportions of Edmondson grade Ⅰ-Ⅱ and TNM stage Ⅰ-Ⅱ were lower than those in the low expression group(P<0.05). The survival rate of the high expression group was lower than that of the low expression group(P<0.05). Multivariate analysis showed that Edmondson grade, TNM stage, intrahepatic metastasis, hepatitis B history, postoperative recurrence and high expression of ZEB1-AS1 were independent factors affecting the clinical prognosis of HCC patients(P<0.05). Conclusion This study confirms that the high expression of ZEB1-AS1 is associated with the malignant degree of HCC, which can be used as an independent risk factor for predicting the clinical prognosis of HCC patients and a potential therapeutic target for HCC.
作者
时北京
杨彦平
鱼永宾
郭辉
SHI Bei-jing;YANG Yan-ping;YU Yong-bin;GUO Hui(General Surgery Department,the Hospital of Gaoling District,Xi'an 710200;Neurosurgery Department,Xi'an Central Hospital,Xi'an 710003,China)
出处
《临床医学研究与实践》
2019年第8期5-6,9,共3页
Clinical Research and Practice
