摘要
目的探讨非编码RNA(LncRNA)盒锌指蛋白1反转录本1(ZEB1-AS1)在卵巢癌(OC)恶性表型调控中的作用。方法选取60例OC患者的癌组织和癌旁组织,利用GSE19804数据集分析ZEB1-AS1在OC组织和正常肺组织中的表达。将细胞分为Control组、shNC组、shZEB1-AS1组、miR-200c mimic组、shZEB1-AS1+miR-200c inhibitor组。qRT-PCR检测ZEB1-AS1或miR-200c在不同组织、正常卵巢上皮细胞与不同OC细胞中的表达;MTT法和Transwell法分别检测细胞增殖、侵袭能力,Western blot检测多种生物标志物在细胞中的表达;用荧光素酶活性检测miR-200c与ZEB1-AS1或Wnt的相互作用。结果OC组织中ZEB1-AS1表达明显高于癌旁组织(P<0.05);与正常卵巢内皮细胞系IOSE80相比,ZEB1-AS1在三个OC细胞系中均过表达(P<0.05),而miR-200c表达下调(P<0.05);miR-200c与ZEB1-AS1或Wnt存在靶向作用关系;与shNC组相比,shZEB1-AS1组和miR-200c mimic组细胞的增殖能力、侵袭能力、MMP2、MMP9、Wnt、β-actin蛋白表达均明显降低;与shZEB1-AS1组相比,shZEB1-AS1+miR-200c inhibitor组细胞的增殖能力、侵袭能力、MMP2、MMP9、Wnt、β-actin蛋白表达均明显升高(P<0.05)。结论ZEB1-AS1可能通过调控miR-200c抑制Wnt/β-catenin信号通路,从而抑制卵巢癌细胞的增殖和侵袭。
Objective To investigate the role of non-coding RNA(LncRNA)zinc finger protein 1 reverse transcript 1(ZEB1-AS1)in the regulation of malignant phenotype in ovarian cancer(OC).Methods The cancer tissues and adjacent tissues of 60 patients with OC were selected.The expression of ZEB1-AS1 in OC tissues and normal lung tissues was analyzed by GSE19804 data set.The cells were divided into control group,shNC group,shZEB1-AS1 group,miR-200c mimic group and shZEB1-AS1+miR-200c inhibitor group.QRT-PCR was used to detect the expression of ZEB1-AS1 or miR-200c in different tissues,normal ovarian epithelial cells and different OC cells.The ability of cell proliferation and invasion was detected by MTT method and Transwell method respectively,and the expression of many biomarkers in cells was detected by Western blot.The interaction between miR-200c and ZEB1-AS1 or Wnt was detected by luciferase activity.Results The expression of ZEB1-AS1 in OC tissues was significantly higher than that in adjacent tissues(P<0.05).Compared with normal ovarian endothelial cell line IOSE80,ZEB1-AS1 was overexpressed in all three OC cell lines(P<0.05),while miR-200c was down-regulated(P<0.05).There was a targeted relationship between miR-200c and ZEB1-AS1 or Wnt.Compared with shNC group,the proliferation and invasion of cells,the expression of MMP2,MMP9,Wnt andβ-actin in shZEB1-AS1 group and miR-200c mimic group were significantly lower than those in shZEB1-AS1 group,while the proliferation and invasion of cells,the expression of MMP2,MMP9,Wnt andβ-actin in shZEB1-AS1+miR-200c inhibitor group were significantly higher than those in shZEB1-AS1+miR-200c inhibitor group.Conclusion ZEB1-AS1may inhibit the proliferation and invasion of ovarian cancer cells by regulating miR-200c to inhibit Wnt/β-catenin signal pathway.
作者
刘翠翠
张颖
王敏娜
关婷
LIU Cui-cui;ZHANG Ying;WANG Min-na;GUAN Ting(Department of Obstetrics and Gynecology,Fushun Central Hospital,Fushun 113006;College of Pharmacy,Liaoning Institute of Science and Technology,Benxi 117004,China)
出处
《解剖科学进展》
CAS
2022年第4期388-393,共6页
Progress of Anatomical Sciences
关键词
长链编码RNA
卵巢癌
增殖
侵袭
long chain coding RNA
ovarian cancer
proliferation
invasion
