摘要
目的 研究CBA/N、nude、SCID小鼠建立人肝癌模型和重建SCID免疫的特点 ,初步探讨B淋巴细胞及其抗体对肝癌细胞生长的排斥作用。方法 将体外培养的人肝癌细胞接种到B细胞缺陷的CBA/N ,T细胞缺陷的BALB/c nu ,T、B细胞缺陷的SCID小鼠及免疫重建的SCID小鼠中 ,观察瘤细胞生长特点 ;取实验小鼠血清 ,测定荷瘤鼠抗体Ig含量的变化 ,取小鼠血清与肝癌细胞作抗原抗体凝集试验。结果 CBA/N和用BALB/c小鼠外周血淋巴细胞重建的SCID(B PBL SCID)小鼠不成瘤 ,nude、SCID和用CBA/N外周血淋巴细胞重建的SCID(C PBL SCID)小鼠 10 0 %成瘤 ;有B淋巴细胞的实验鼠 (包括B PBL SCID)测到的Ig达到mg/ml水平 ,血清能与肝癌产生凝集反应。 结论 SCID小鼠是建立肿瘤模型和免疫重建及其肿瘤免疫研究的理想实验动物 ;B淋巴细胞及其产生的抗体在抗肿瘤中起着重要的作用。
Objective To investigate the characteristics of established hepatocarcinoma models in CBA/N, nude, SCID mice and reconsituted SCID mice and to appraise preliminarily the rejection effect of B lymphocytes and antibody produced on cancer cell growth in vivo.Methods The human hepatocarcinoma cells cultured in vitro were inoculated into B lymphocyte deficient CBA/N mice, T lymphocyte deficient nude mice, combined T and B lymphocytes deficient SCID mice, immunoreconstituted SCID mice with BALB/c mice peripheral blood lymphocytes(B-PBL-SCID), immunoreconstituted SCID mice with CBA/N mice peripheral blood lymphocytes (C-PBL-SCID) and their growths were observed. The sera immunoglobulin concentrations in BALB/c and B-PBL-SCID mice were determined. The serum agglutination of antigen-antibody in the mice with hepatocarcinoma cells (target cells) was tested.Results There were no cancer growths in CBA/N and B-PBL-SCID mice, but were 100% cancer growths in SCID, nude and C-PBL-SCID mice. The immunogloblin reached the level of mg/ml in BALB/c and B-PBL-SCID mice. The sera of experimental mice having B lymphocytes could agglutinate the cancer antigen, and the agglutination of sera in BALB/c mice was more intense than that in B-PBL-SCID mice. Conclusion SCID mouse is an ideal hepatocarcinoma animal model for studying tumor immunity; B lymphocytes and the antibodies produced play important role in the rejection of carcinoma cells.
出处
《肝脏》
2003年第4期21-23,共3页
Chinese Hepatology
关键词
SCID
小鼠
的免疫重建
B淋巴细胞
抗癌作用
肝癌
Hepatocarcinoma cell
Inoculation
Immunodeficient mice
SCID immunoreconstitution
Antibody
