摘要
目的 探讨抗氧化剂N_乙酰半胱氨酸 (NAC)对急性坏死性胰腺炎 (ANP)大鼠胰腺组织一氧化氮 (NO)及丙二醛(MDA)的影响。方法 雄性SD大鼠 114只 ,随机分成正常对照组 (C组 ,n =3 0 )、急性胰腺炎组 (A组 ,n =42 )和NAC干预组 (N组 ,n= 42 )。A组分 2次腹腔内注射 8%L_精氨酸 (L_Arg) 1 2mg/g诱导ANP ;C组同法腹腔内注射等量生理盐水 ;N组先提前 1小时 (h)腹腔内注射 0 5mol/L的NAC 0 0 5mg/g ,然后同A组方法诱导ANP。在首次注射L_Arg后于 6h、12h、2 4h、3 6h、48h、72h分批处死大鼠 ,观察大鼠胰腺组织NO和MDA水平及胰腺病理变化。结果 N组各时点NO和MDA水平均较A组明显降低 (均P <0 0 1或P<0 0 5) ,N组 12h、2 4h、3 6h、48h、72h的胰腺病理改变较A组的明显减轻 (均P <0 0 1或P <0 0 5)。结论 NAC可降低胰腺组织NO、MAD水平 ,减轻了实验性ANP大鼠的胰腺损害 。
Objective To observe the influence of N_acetylcysteine(NAC) on nitric oxide(NO) and malona ldehyde(MDA) in rat's pancreatic tissue with acute necrotizing pancreatitis(ANP) . Methods 114 SD male rats were randomly divided into control group (group C,n=30),acu te p ancreatitis group (group A,n=42) and NAC intervention group (group N, n=42). In gr oup A,SD rats were injected twice intraperitoneally with 8% L_Arginine(2×1 2 m g/g) in an interval of 1 hour for ANP.In group C,SD rats received the same amoun t of saline at the same time.In group N,0 5 mol/L NAC (0 05 mg/g) was administ ered intraperitoneally 1 hour befor L_Arginine injection.Animals were killed at 6,12,24,36,48,72 hours after the first L_Arginine injection.The level of NO and MDA in rat's pancreatic tissue sample of each group were assayed and pathologic changes of pancreatic tissue were observed under microscopes. Results①In group N,the level of NO and MDA were lower than those in the group A(P <0 05 or P<0 01).②In group N,the pathologic changes of pancreatic tiss ue at 12 h,24 h,36 h,48 h and 72 h after the injection of L_Arginine were significantly milder than those in group A(P<0 05 or P<0 01). Conclusion NAC treatment can decrease NO and MDA of pancreatic tissue,and prevent the pancr eatic histologic damage in Arg_induced ANP.
出处
《胃肠病学和肝病学杂志》
CAS
2003年第6期527-529,共3页
Chinese Journal of Gastroenterology and Hepatology
