摘要
目的复制伴高脂血症性急性坏死性胰腺炎(HAP)大鼠模型,观察巨噬细胞移动抑制因子(MIF)在HAP发病中的变化。方法 50只雄性SD大鼠随机分为4组:对照组(C组,n=5)、胰腺炎组(ANP组,n=15)、高脂组(HL组,n=15)、伴高脂血症性胰腺炎组(HAP组,n=15),采用高脂饲料喂养4周复制大鼠高脂血症模型,采用逆行胰胆管注射3.5%牛磺胆酸钠复制胰腺炎模型,于术后4、9和24 h各处死5只大鼠,观察胰腺组织病理改变并评分,检测血清淀粉酶(Amyl)及三酰甘油(TG)水平,ELISA法测定血清MIF及TNF-α水平。结果高脂饲料组大鼠的血清TG水平明显高于基础饲料组(P<0.01);与C组相比,ANP组和HAP组各时点的血清Amyl、MIF、TNF-α水平及胰腺组织病理学评分均明显升高(P<0.01或P<0.05);与ANP组相比,HAP组TNF-α水平只在24 h明显升高(P<0.01);HAP组各时点MIF水平与ANP组相比无明显升高(P>0.05);ANP组和HAP组胰腺组织病理学评分与血清MIF、TNF-α水平均呈正相关(P<0.05)。结论 HAP发病早期血清MIF表达增加,提示MIF参与了HAP的发生发展过程。
【Objective】 Established a rat model with hyperlipidemia and acute necrotizing pancreatitis（HAP） in purpose to observe the changes of migration inhibitory factor（MIF） in the pathogenesis of HAP.【Methods】 Fifty male SD rats were randomly divided into 4 groups： Control group（group C,n =5）,ANP group（n =15）,HL group（n =15）,HAP group（n =15）.Hyperlipemia rat model was established by feeding a high fat diet for 4 weeks.AP rat model was established by retrograde injection of 3.5% sodium taurocholate into the biliopancreatic duct.Rats were sacrificed at 4 h,9 h,24 h later respectively.Pancreatic histopathology was detected and evaluated,the serum diastase（Amyl） and triglyceride（TG） were measured,the levels of serum MIF and TNF-α were determined by ELISA.【Results】 The serum TG of rats fed with high fat feed was higher than that of rats fed with basic feed（P 0.01）.Compared with group C,serum Amyl,MIF,TNF-α and pancreatic histopathological scores in both ANP group and HAP group were significantly higher（P 0.01 or P 0.05）.Compared with ANP group,serum TNF-α levels were significantly higher only at 24 h（P 0.01）,serum MIF levels in HAP group had no changes.Both serum MIF levels and TNF-α levels presented a positive correlation with the pancreatic histopathological scores（P 0.05）.【Conclusions】 Serum MIF increases early in HAP,suggesting that MIF is involved in the occurrence and development of HAP.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2012年第26期30-33,共4页
China Journal of Modern Medicine
