摘要
目的优化链脲佐菌素(streptozotocin,STZ)诱导的C57BL/6J糖尿病小鼠模型。方法采用单用不同剂量STZ(180 mg·kg^(-1)单次给药和275 mg·kg^(-1)均分5次,每次55 mg·kg^(-1),连续5 d)或4周高脂饮食联合不同剂量STZ(50 mg·kg^(-1)单次给药;100 mg·kg^(-1)单次给药;150 mg·kg^(-1)单次给药;200 mg·kg^(-1)均分2次给药,间隔72 h),建立糖尿病小鼠模型,检测各组小鼠空腹血糖、体质量、日饮水量及日进食量,比较各组造模成功率及稳定性。结果STZ 180 mg·kg^(-1)单次给药、高脂饮食4周+STZ 150 mg·kg^(-1)单次给药、高脂饮食4周+STZ 200 mg·kg^(-1)均分2次给药得到的糖尿病小鼠模型,其高血糖的持续时间较长且稳定。结论STZ 180 mg·kg^(-1)单次给药是较为理想的1型糖尿病模型;4周高脂饮食联合STZ 150mg·kg^(-1)单次给药或200 mg·kg^(-1)均分2次给药均是较为理想的2型糖尿病模型。
OBJECTIVE To optimize the C57 BL/6 J diabetic mice model induced by streptozotocin(STZ). METHODS Different diabetic mice model were established with different doses of STZ intraperitoneal administration(180 mg·kg-1 single dose and 275 mg·kg-1 averaged by 5 d, 55 mg·kg-1 each day) and 4-week high-fat diet combined with different doses of STZ(50 mg·kg-1 single dose, 100 mg·kg-1 single dose, 150 mg·kg-1 single dose and 200 mg·kg-1 for two times at 72 h intervals).Fasting blood glucose, weight, water-drinking and food-consumption were determined, the modeling rate and stability of each group were compared. RESULTS Diabetic model induced by STZ 180 mg·kg-1 single dose, four weeks high-fat diet combined with STZ 150 mg·kg-1 single dose or 200 mg·kg-1 for two times administration were more stable in the long run.CONCLUSION STZ 180 mg·kg-1 single dose is a suitable type 1 diabetic model, meanwhile, four weeks high-fat diet combined with STZ 150 mg·kg-1 single dose or 200 mg·kg-1 for two times administration are appropriate type 2 diabetic model.
作者
张吟
黄丹丹
张淑芬
许秋霞
李营营
ZHANG Yin;HUANG DANDan;ZHANG Shufen;XU Qiuxia;LI Yingying(Department of Pharmacy,The Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China;College of Pharmacy,Fujian Medical University,Fuzhou 350108,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2019年第6期655-660,共6页
Chinese Journal of Modern Applied Pharmacy
基金
国家卫生计生委共建科学研究基金(WKJ-FJ-13)
福建省卫生系统中青年骨干人才培养项目(2015-ZQN-ZD-23)
