摘要
目的观察2型糖尿病小鼠模型血糖变化规律,探讨餐后2 h血糖作为血糖干预评价点的可行性。方法选取健康C57BL/6小鼠,用高脂饮食加链脲佐菌素(STZ)诱导2型糖尿病(T2DM)实验组,并设立正常对照。分别测定两组连续空腹血糖及连续随机血糖,分析小鼠血糖规律,评估餐后2 h血糖作为T2DM小鼠血糖干预评价点的科学性;通过长期观察糖尿病小鼠餐后2 h血糖波动,验证餐后2 h血糖干预评价点的稳定性。结果T2DM小鼠连续空腹血糖显示,餐后空腹2~3 h期间血糖变化幅度相对稳定,禁食超过4 h后,T2DM组小鼠血糖继续大幅度降低,而正常组却能缓慢上升,并保持血糖相对稳定;7周内T2DM小鼠餐后2 h血糖波动显示,餐后2 h血糖稳定。结论餐后2 h血糖能稳定地反映T2DM小鼠模型的血糖变化规律,具有较好的血糖干预实验评价应用价值。
Objective To observe changes of blood glucose in mice with type 2 diabetes mellitus(T2DM),and to explore the feasibility of using 2-hour postprandial blood glucose as an evaluation point of blood glucose intervention.Methods T2DM mice induced by a high-fat diet and streptozotocin(STZ)were the experimental group and normal C57BL/6 mice were used as the control group.Continuous fasting blood glucose and continuous random blood glucose were measured in the two groups,and the blood glucose levels in the mice were analyzed to evaluate the use of 2-hour postprandial blood glucose as an evaluation point of blood glucose intervention in T2DM mice.The stability of the 2-hour postprandial blood glucose evaluation point was verified by the long-term observation of 2-hour postprandial blood glucose fluctuation in diabetic mice.Results The continuous fasting blood glucose of T2DM mice was significantly reduced after fasting for more than 4 hours.In contrast,blood glucose changes in T2DM mice at 2-3 hours after eating a meal were relatively stable,which might reflect the stability of the model over a long period.For 7 weeks,the 2-hour postprandial blood glucose level of T2DM mice showed it was stable at 2 hours after meals.Conclusions The 2-hour postprandial blood glucose level reflects the stable blood glucose change in T2DM mice,which might have value for the evaluation of blood glucose intervention experiments.
作者
蒲瑞阳
史典
刘莎
程宁
PU Ruiyang;SHI Dian;LIU Sha;CHENG Ning(Key Laboratory of Preclinical Study for New Drugs of Gansu Province,Basic Medical College,Lanzhou University,Lanzhou 730000,China)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2020年第2期224-229,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
兰州大学“一带一路”专项项目资助(2018ldbrzd008)
国家自然科学基金(81673248)。
关键词
2型糖尿病模型
C57BL/6小鼠
空腹血糖
随机血糖
餐后2
h血糖
type 2 diabetic model
C57BL/6 mice
fasting plasma glucose
random blood glucose
2-hour postprandial blood glucose
