摘要
目的观察白僵蚕多糖对2型糖尿病小鼠的抗氧化作用。方法从31只SPF级雄性小鼠中随机取7只作为空白组,其余24只小鼠制备2型糖尿病模型,造模期间死亡3只。将21只成功建模小鼠随机分为3组,模型组、白僵蚕多糖组、二甲双胍组各7只。白僵蚕多糖组予白僵蚕多糖溶液400 mg/kg,二甲双胍组予盐酸二甲双胍溶液200 mg/kg,模型组予0.9%氯化钠注射液0.2 mL/10 g,空白组予0.9%氯化钠注射液0.2 mL/10 g,均每日灌胃1次,连续给药28天。观察各组小鼠肝脏病理学变化;比较各组小鼠体质量、空腹血糖(FPG)、脏器指数(脾脏指数、肾脏指数)、氧化指标[总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)]变化。结果空白组肝细胞排列规整,细胞范围清晰,没有水肿、炎症等。模型组细胞排列紊乱,绝大多数肝细胞已出现弥漫性水肿变性和轻度脂肪变性,细胞组织疏松、空泡化较严重,有许多大小不等整齐的小圆空泡,炎症程度一般较轻。白僵蚕多糖组肝细胞结构比较均匀完整,有轻度松散,细胞表面有少量大小深浅不等的小空泡形成和轻微的水肿,细胞间有脂肪变性,无炎症。二甲双胍组肝细胞较为整齐,细胞略有空泡变性,细胞体中度肿大,无炎症。与空白组比较,模型组小鼠体质量降低(P<0.05),FPG水平升高(P<0.05);与模型组比较,白僵蚕多糖组、二甲双胍组小鼠体质量升高(P<0.05),FPG水平降低(P<0.05);白僵蚕多糖组与二甲双胍组小鼠体质量、FPG水平比较差异均无统计学意义(P>0.05)。与空白组比较,模型组脾脏指数、肾脏指数均升高(P<0.05);与模型组比较,白僵蚕多糖组、二甲双胍组脾脏指数、肾脏指数均降低(P<0.05);白僵蚕多糖组与二甲双胍组脾脏指数、肾脏指数比较差异无统计学意义(P>0.05)。与空白组比较,模型组T-SOD、GSH含量均降低(P<0.05),MDA含量升高(P<0.05);与模型组比较,白僵蚕多糖组、�
Objective To investigate the antioxidant effect of Bombyx Batryticatus Polysaccharide(BBP)on mice with type 2 diabetes mellitus(T2DM).Methods A total of 30 Kunming male mice in the specific pathogen-free(SPF)level were used.Seven randomly selected mice were treated with blank control(control group),and the remaining 23 were subjected to the modeling of T2DM.Two mice died during the modeling period.Twenty-one T2DM mice were randomly divided into the model group,BBP group and metformin group,with 7 mice per group.Mice in the BBP group and metformin group were administrated with 400 mg/kg BBP solution and 200 mg/kg metformin solution by oral gavage once daily for 28 days,respectively.Mice in the model group and control group were similarly given 0.2 mL/10 g 0.9%NaCl solution.Pathological changes in mouse liver were observed.Body mass,fasting plasma glucose(FPG),organ indexes(spleen index,kidney index)and oxidation stress indexes(total superoxide dismutase[T-SOD],malondialdehyde[MDA]and glutathione[GSH])were compared.Results Mice in the control group presented a regular arrangement of liver cells with a clear margin,and edema and inflammation were not observed.Mice in the model group presented an irregular arrangement,diffuse edema degeneration and mild steatosis in the majority of liver cells,loose cellular tissue,severe vacuolization with many small round vacuoles of varying sizes,and mild inflammation.Mice in the BBP group presented uniform and completely-shaped liver cells,slightly loose cellular tissues,a small number of small round vacuoles of varying sizes on cell surface,mild edema,steatosis and no inflammation.Mice in the metformin group presented a regular arrangement of liver cells,mild vacuolization,moderate cell body swelling and no inflammation.Compared with those of the control group,mice in the model group presented significantly lower body mass and higher FPG(both P<0.05).Compared with those of the model group,mice in the BBP group and metformin group presented significantly higher body mass and lowe
作者
姚雨辰
姚思佳
竺世骄
张杨
项嘉琪
付琳
龙娟
YAO Yuchen;YAO Sijia;ZHU Shijiao;ZHANG Yang;XIANG Jiaqi;FU Lin;LONG Juan(the First Clinical College of Changsha Medical University,Changsha,Hunan 410219;School of Basic Medicine,Changsha Medical University,Changsha,Hunan 410219)
出处
《河北中医》
2024年第3期439-442,共4页
Hebei Journal of Traditional Chinese Medicine
基金
2021年度长沙医学院大学生创新创业训练计划项目(长医教〔2021〕47号-16)
大学生创新创业训练计划项目(编号:S202110823016)。
关键词
糖尿病
2型
白僵蚕
抗氧化
动物实验
Type 2 diabetes mellitus
Bombyx Batryticatus
Antioxidation
Animal experiment
