摘要
目的:研究姜黄素(Cur)对过氧化氢(H2O2)诱导的血管内皮细胞ECV304的作用及可能机制;方法:胎盘兰计数法分组观察在不同的时间点加入姜黄素后对H2O2诱导的ECV304细胞氧化损伤的作用.流式细胞仪分析细胞周期及细胞凋亡率的变化,试剂盒检测一氧化氮(NO)、丙二醛(MDA)含量及超歧化物氧化酶(SOD)、谷胱甘肽还原酶(GR)活性的变化.结果:姜黄素0～100 μmol·L-1与H2O2 500 μmol·L-1同时作用5 h,随姜黄素浓度升高细胞存活率升高;25～100 μmol·L-1姜黄素预先作用1 h,再换为H2O2 500 μmol·L-1作用4 h,细胞存活率明显下降;H2O2500 μmol·L-1作用4 h,再加入25～100 μmol·L-1姜黄素作用1 h,细胞存活率也升高.姜黄素对H2O2诱导的细胞凋亡没有明显的影响,但同时作用组、H2O2先作用组S期细胞所占比例升高,姜黄素先作用组S期细胞所占比例下降.同时作用组、H2O2先作用组的SOD、NO、GR水平相对升高,MDA水平下降,姜黄素先作用组的SOD、NO、GR水平相对下降,MDA水平升高.结论:姜黄素对H2O2诱导的血管内皮细胞ECV304有双重效应,即有保护效应也有细胞毒效应,与其作用时间点有关.
AIM： To observe the effect and mechanisms of curcumin to hydrogen peroxide induced oxidative vascular endothelial cells ECV3O4 damage. METHODS： Estimate cell survival rates of curcumin to H2O2 induced oxidative ECV3O4 damage by trypan blue staining. The observed the change of cell cycle and cell apoptosis rates by FACS. Detect the expression of NO, MDA contents and SOD, GR activity by test kits. RESULTS： When added simultaneously with 500 μmol·L^-1 curcumin （0- 100 μmol·L^-1 ） for 5 h effectively protected cells form oxidative damage. However, when the cells were pretreated with curcumin （25 - 100 μmol·L^-1 ） for lh before H2O2 exposure for 4 h, curcumin was unable to inhibit H2O2- induced cell damage. Instead; when pretreated with H2O2 for 4h and then added curcumin （25 - 100μmol·L^-1 ）, cell survival rote also increased. It was no obviously change of cell apoptosis rates by curcumin to H2O2-induced cell damage, but curcumin was found to induce Sphase cell cycle arrest in curcumin together with H2O2 group and post-treatment with curcumin group, and adversely in pretreatment with curcumin group. CONCLUSION： The curcumin has dual action to hydrogen peroxide induced oxidative vascular endothelial cells damage, cytoprotective and cytotoxic effects, correlated different chronologicaol order treated ECV3O4 which is with curcumin.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第12期1376-1380,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
