摘要
目的研究间充质干细胞(MSC)对小鼠的急性毒性作用。方法根据ICR小鼠预实验结果分为3组:对照组(生理盐水)、高、低2个剂量实验组(间充质干细胞浓度分别为2×108,1×107cells·kg-1),每组20只,雌雄各半。单次尾静脉给药,给药体积为20 m L·kg-1,分组观察小鼠对MSC的反应。结果在各个时间点,小鼠的平均体重,实验组与对照组比较差异均无统计学意义。与对照组比较,低、高2个剂量实验组雄性小鼠脾、肺重量及其系数明显升高,高剂量实验组雌性小鼠肺系数明显升高。结论 MSC无明显的毒副作用,MSC在肺和脾等部位有滞留。
Objective To observing the acute toxicity of mesenchymal stem cell（ MSC） in the ICR mice. Methods The ICR mice were divided three groups based on the preliminary experiment, including high- dose of MSC group（ 2. 0 × 10^8cells·kg^- 1）,low- dose of MSC group（ 1. 0 × 10^7 cells · kg^- 1） and control group（ normal saline）,each group has 20 mice（ male and female,1∶ 1）. And then all the cells were tested respectively by the classic methods of acute toxicity in ICR mice.The reactions of mice to MSC was observed after single dosing of20 mL·kg^- 1MSC through tail vein administration. Results There are no difference on the weight in three groups. But compared with the control group,the weight and weight index of the spleen and lung of the male mice in test- high and test- low dose group increased obviously.Besides,weight index of lung of the female mice in high- dose group increased evidently. Conclusion There are no obvious adverse drug reaction after administration MSC except that MSC has a stranded in some parts such as lung and spleen.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第16期1645-1647,共3页
The Chinese Journal of Clinical Pharmacology
基金
国家重大新药创制专项基金资助项目(2014ZX09101042
2014ZX09101042-003)
关键词
间充质干细胞
表型鉴定
急性毒性
ICR小鼠
脏器系数
mesenchymal stem cell
phenotypic identification
acute toxicity
ICR mice
organ coefficient
