摘要
目的:观察电针"夹脊"穴预处理对心肌缺血再灌注损伤(MIRI)大鼠的心肌保护作用及对细胞色素P450(CYP450)信号通路蛋白表达的影响。方法:Wistar大鼠随机分为假手术组、模型组、夹脊组、内关组、阳陵泉组、曲池组,每组10只。采用左冠状动脉结扎法复制MIRI大鼠模型。各电针预处理组电针相应穴位,每次30min,每日1次,连续针刺7d。HE染色法观察各组大鼠心肌组织病理形态,透射电镜下观察心肌细胞超微结构,Western blot法检测心肌CYP450相关分子CYP1A1、CYP2B2、CYP2C11、CYP4A2蛋白的表达水平。结果:模型组缺血区心肌纤维肿胀、紊乱,可见局灶性坏死区,心肌间质有大量炎性细胞浸润。夹脊组和内关组均可见心肌纤维轻度肿胀,散见部分坏死,间质出血,与模型组比较,炎性细胞浸润明显减轻。透射电镜下可见模型组肌原纤维排列紊乱,线粒体结构模糊,肿胀、空泡化。夹脊组和内关组心肌纤维结构较清晰,线粒体数量和结构无明显异常。与假手术组比较,模型组心肌CYP1A1、CYP2B2蛋白表达水平均显著增高(P<0.05)。与模型组比较,夹脊组、内关组、阳陵泉组心肌CYP1A1、CYP4A2蛋白表达水平均显著降低(P<0.01),夹脊组和内关组心肌CYP2B2、CYP2C11蛋白表达水平显著增高(P<0.01)。与夹脊组比较,曲池组心肌CYP1A1、CYP4A2蛋白表达水平显著升高(P<0.05),CYP2B2、CYP2C11蛋白表达水平显著降低(P<0.05)。结论:电针"夹脊"穴和"内关"穴预处理均能够上调CYP450表氧化酶蛋白表达水平,下调CYP450ω-羟化酶蛋白表达水平,起到心肌保护作用。
Objective To investigate the effect of'Jiaji'(EX-B2)-electroacupuncture(EA)preconditioning on structural changes of myocardium and expression of cytochrome P450(CYP450)enzymes in acute myocardial ischemia-reperfusion injury(MIRI)rats.Methods Sixty male Wistar rats were randomly divided into sham operation,model,EX-B2,Neiguan(PC6),Yanglingquan(GB34)and Quchi(LI11)groups(n=10 in each group).The MIRI model was established by occlusion of the descending anterior branch of the left coronary artery for 30 min,followed by reperfusion for 30 min.EA preconditioning(2 Hz/100 Hz,1 mA)was respectively applied to EX-B2,PC6,GB34,and LI11 for 30 min,once daily for 7 days.Morphological changes of myocardium were observed by H.E.staining.The ultrastructure of myocardial cells was observed by transmission electron microscope(TEM).The protein expression levels of myocardial CYP450(CYP1 A1,CYP2 B2,CYP2 C11 and CYP4 A2)were detected by Western blot.Results In MIRI rats,myocardial pathological changes as swollen,disorganized arrangement,and partially ruptured myocardial fibers with unclear limit and necrosis and infiltration of lots of inflammatory cells under microscope,and focal myofilament degeneration,dissolution and necrosis with interstitial edema,vague mitochondria with swelling and vacuolation,ruptured and disorganized arrangement of crests under TEM were found in the ischemic area,which was obviously milder in rats of both EX-B2 and PC6 groups.After modeling,the expression of myocardial CYP1 A1 and CYP2 B2 protein were significantly up-regulated in the model group relevant to the sham operation group(P<0.05).In EA preconditioning groups,the expression levels of CYP1 A1 and CYP4 A2 proteins in the EX-B2,PC6 and GB34 groups were obviously down-regulated(P<0.01),and those of CYP2 B2 and CYP2 C11 proteins in both EX-B2 and PC6 groups were markedly up-regulated relevant to the model group(P<0.01).Compared with the EX-B2 group,the expression levels of CYP1 A1,CYP4 A2 proteins in the LI11 group were significantly up-regulated(P<0.05
作者
李洋
于慧娟
张昌云
谭奇纹
崔华峰
LI Yang;YU Hui-juan;ZHANG Chang-yun;TAN Qi-wen;CUI Hua-feng(College of Acupuncture-moxibustion and Tuina,Shandong University of Traditional Chinese Medicine,Jinan250011,China;Department of Preventive Treatment ofDisease,3Department of Acupuncture and Moxibustion,the Affiliated Hospital of Shandong University of Traditional ChineseMedicine,Jinan250011)
出处
《针刺研究》
CAS
CSCD
北大核心
2019年第4期248-252,263,共6页
Acupuncture Research
基金
国家自然科学基金项目(No.81373722)
山东省自然科学基金联合专项(No.ZR2014HL100)
关键词
电针
预处理
心肌缺血再灌注
细胞色素P450
Electroacupuncture
Preconditioning
Myocardial ischemia-reperfusion
Cytochrome P450
