摘要
目的:观察电针预处理对心肌缺血再灌注损伤(MIRI)大鼠法尼酯衍生物X受体(FXR)/小异二聚体配体(SHP)凋亡通路及凋亡诱导因子(AIF)和热休克蛋白70(HSP70)的影响,探讨电针预处理改善MIRI的作用机制。方法:Wistar大鼠随机分为正常对照组、假手术组、缺血再灌注组、电针预处理组,每组10只。采用结扎大鼠左冠状动脉前降支法建立在体MIRI大鼠模型。电针预处理组取"内关""关元""足三里"给予电针干预,每次20 min, 1次/d,连续7 d。通过BL-420S生物机能实验系统对大鼠进行心功能评价,酶联免疫法检测大鼠血清TNF-α和IL-6的含量,荧光定量PCR法检测大鼠心肌组织中FXR、SHP、AIF及HSP70 mRNA相对表达量。结果:与正常对照组、假手术组比较,缺血再灌注组大鼠左心室舒张末压(LVEDP)、血清TNF-α和IL-6的含量及心肌组织中FXR、SHP、AIF及HSP70 mRNA相对表达量均显著升高(P<0.05),左心室收缩压(LVSP)、左室内压最大上升/下降速率(±dp/dt_(max))显著降低(P<0.05)。与缺血再灌注组比较,电针预处理组大鼠LVEDP、血清TNF-α和IL-6的含量及心肌组织中FXR、SHP及AIF的mRNA相对表达量均显著降低(P<0.05),LVSP、±dp/dt_(max)、HSP70 mRNA相对表达量显著升高(P<0.05)。结论:电针预处理可以对MIRI起到预防保护作用,其机制可能与电针预处理调节FXR/SHP信号通路抑制心肌细胞凋亡有关。
Objective To observe the effect of electroacupuncture(EA) preconditioning on left-cardiac function, contents of serum TNF-α and IL-6 and expression of myocardial farnesoid X receptor(FXR), small heterodimer partner(SHP), apoptosis inducing factor(AIF) and heat shock proteins 70(HSP70) genes in myocardial ischemia-reperfusion injury(MIRI) rats, so as to explore its mechanisms underlying improvement of ischemic myocardial injury. Methods Forty male Wistar rats were randomly divided into normal control, sham operation, MIRI model and EA pretreatment groups, with 10 rats in each group. Rats of the sham operation group received exposure of the thorax and heart. The MIRI model was established by occlusion of the anterior descending branch of the left coronary artery(LAD). EA(2 Hz/100 Hz and 1 mA) was applied to bilateral "Neiguan"(PC6), "Zusanli"(ST36) and "Guanyuan"(CV4) for 20 min, once a day for 7 days. The left ventricular end-diastolic pressure(LVEDP), left ventricular systolic pressure(LVSP)and maximal rates of rise and fall of left ventricular pressure(±dp/dt_(max)) were detected, the contents of serum TNF-α and IL-6 were detected by using enzyme-linked immunosorbent assay(ELISA), and the expression of FXR, SHP, AIF and HSP70 apoptotic genes in the myocardial tissue were measured by fluorescent quantitative RT-PCR. Results Compared with the normal control group, the LVEDP, contents of serum TNF-α and IL-6, and the expression levels of myocardial FXR, SHP, AIF and HSP70 mRNAs were significantly increased(P<0.05), while LVSP and ±dp/dt_(max) levels were obviously decreased in the model group(P<0.05). In comparison with the model group, MIRI-induced increases of LVEDP, TNF-α and IL-6 contents, and FXR, SHP and AIF mRNA expression and decreases of ±dp/dt_(max) and LVSP levels were reversed(P<0.05), except HSP70 mRNA expression with significantly increased(P<0.05) in the EA pretreatment group. Conclusion EA pretreatment can protect the left ventricular function of the ischemic heart in MIRI rats, which may be
作者
刘珍珍
黄伟
向丽莉
杨琪琪
薛艺璇
吴松
李佳
LIU Zhen-zhen;HUANG Wei;XIANG Li-li;YANG Qi-qi;XUE Yi-xuan;WU Song;LI Jia(College of Acupuncture-moxibustion and Orthopedics,Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases with Acupuncture and Moxibustion,Wuhan 430061,China;First Clinical College,Hubei University of Chinese Medicine,Wuhan 430061;Department of Acupuncture-moxibustion,Hubei Provincial Hospital of Taditional Chinese Medicine,Wuhan 430061)
出处
《针刺研究》
CAS
CSCD
北大核心
2021年第5期368-374,共7页
Acupuncture Research
基金
国家自然科学基金青年基金项目(No.81704142)
中国科协青年人才托举工程(No.2017QNRC001)
国家中医药管理局岐黄工程项目(No.国中医药人教函[2018]284号)
湖北中医名师工作室项目(鄂卫生计生通报[2018]15号)
湖北省中医院首批昙华林名医、学子培养项目(鄂中医院子[2018]72号)
武汉市中青年医学骨干人才人才培养工程项目(武卫生计生通[2018]116号)。
关键词
心肌缺血
再灌注损伤
电针预处理
细胞凋亡
Myocardial ischemia
Reperfusion injury
Electroacupuncture pretreatment
Apoptosis
