摘要
目的 :原位检测幽门螺杆菌 (Hp)感染时胃黏膜活检标本中肿瘤坏死因子 α(TNF α)和白介素 1 β(IL 1 β)的产生情况。方法 ;采集 1 3例Hp阴性受检者和 32例Hp阳性受检者的胃黏膜活检标本 ,通过放免法、免疫组织化学法观察并比较两组胃黏膜活检标本中细胞因子TNF α和IL 1 β的产生。结果 :胃黏膜活检标本中TNF α和IL 1 β的分泌在Hp阳性组显著高于Hp阴性组 (P <0 .0 1 ) ,但其分泌与胃黏膜的病理分型似无显著相关性 (P >0 .0 5)。TNF α和IL 1 β的组织表达在Hp阳性组也显著高于Hp阴性组 (P <0 .0 5)。结论 :Hp感染可以使局部细胞因子TNF α和IL 1 β产生增加 。
Purpose: To examine the production in situ of tumor necrosis factor-alpha(TNF-α)and interleukin-1beta(IL-1β) in helicobacter pylori(Hp)-associated gastritis. Methods: Biopsy samples were collected from 13 Hp negative subjects and from 32 Hp positive subjects. We examine the concentration of TNF-a and IL-1β by radioimmunoassay (RIA). We also examine the TNF-α, IL-1β expression by using immunohistochemical method. Results: TNF-α and IL-1β were significantly higher in Hp positive group than Hp negative group(P<0.01),but it seemed no significant correlation between TNF-α, IL-1β concentration and the histological type of gastritis(P<0.05).TNF-α,IL-1β expression was significantly higher in Hp positive subjects than Hp negative subjects(P<0.05). Conclusion: Hp infection induced the increasing of TNF-α and IL-1β production, which can play a possible pathogenetic role in Hp-associated gastritis.
出处
《临床消化病杂志》
2003年第6期243-245,共3页
Chinese Journal of Clinical Gastroenterology
