摘要
目的 研究Celecoxib对COX 2高表达人胰腺癌细胞JF 30 5生长和凋亡的影响及作用机制。方法 采用四唑氮蓝 (MTT)比色法检测细胞增殖 ,流式细胞仪测定细胞周期和凋亡 ,酶联免疫吸附试验 (ELISA)检测前列腺素E2 (PGE2 )含量。结果 Celecoxib可明显抑制JF 30 5细胞增殖和诱导其凋亡 ,并且这种增殖抑制作用能被PGE2 拮抗。结论 Celecoxib通过COX 2和PGE2 途径抑制JF 30 5细胞生长和诱导其凋亡 ;Celecoxib可能是COX 2高表达胰腺肿瘤的一种有效的化学治疗和化学预防药物。
Objective To evaluate the effects and mechanisms of celecoxib in inducing proliferation inhibition and apoptosis on human pancreatic carcinoma cells. Methods The anti-proliferative effect was measured by using Methabenzthiazuron (MTT) assay. Cell cycle and apoptosis were analyzed by using flow cytometry. The prostaglandin E 2 (PGE 2) levels in the supernatant of cultured pancreatic carcinoma cells were quantitated by enzyme-linked immunoabsordent assay (ELISA).Results Celecoxib suppressed the production of PGE 2 and inhibited growth of JF-305 cells; and the anti-proliferative effect of celecoxib could be abolished by addition of PGE 2. Celecoxib induced proliferation inhibition and apoptosis by G 1-S cell cycle arrest.Conclusion Cyclooxygenase-2 (COX-2) specific inhibitor celecoxib inhibits proliferation and induces apoptosis of human pancreatic carcinoma cells via suppression of PGE 2 production in vitro.
出处
《肿瘤防治研究》
CAS
CSCD
2003年第6期480-482,共3页
Cancer Research on Prevention and Treatment
