摘要
目的:观察环氧化酶-2(COX-2)选择性抑制剂塞来昔布对化学致癌剂7,12-二甲基苯蒽(DMBA)化学诱发的大鼠乳腺癌形成的影响.方法:DMBA油剂灌胃复制大鼠乳腺癌模型,90只大鼠分为3组,单纯诱癌组作为阴性对照、三苯氧胺组作为阳性对照、观察塞来昔布对大鼠乳腺肿瘤发生率和bcl-2,VEGF蛋白表达的影响.结果:三苯氧胺组(48.2%)和塞来西布组(50.0%)肿瘤发生率低于单纯诱癌组(85.7%,分别为P=0.003,P=0.004).塞来昔布组VEGF蛋白表达率(42.9%)低于单纯诱癌组(79.2%,P=0.023),和三苯氧胺组(46.2%)无差异(P=0.863).塞来昔布组bcl-2蛋白表达率(42.9%)低于单纯诱癌组(83.3%,P=0.010),低于三苯氧胺组(53.9%),但无差异(P=0.568).结论:塞来昔布能抑制DMBA诱发的大鼠乳腺癌的发生、发展,下调bcl-2和VEGF蛋白表达可能是其机制之一.
AIM： To evaluate the chemopreventive effect of cyclooxygenase-2 （ COX-2 ） specific inhibitor, celecoxib, on chemically induced breast cancer in rats, and its mechanisms. METHODS： 7, 12- dimethylbenz anthracene （DMBA） was irrigated into the stomach of SD female rats to build breast cancer model. A total of 90 rats were divided into 3 groups： control group, tamoxifen group and celecoxib group. The occurrence rate of breast cancer, and the effect of celecoxib on the expressions of bel-2 and VEGF were detected by immunohistochemical SP method. RESULTS ： The incidence of tamoxifen group （48.2%） and celecoxib group （50.0%） were lower than that of control group （85.7%）, with statistical difference （ P = 0. 003, P =0.004, respectively）. The positive rate of VEGF in celeeoxib group （42.9%） was lower than that in control group （79.2%, P=0.023） , while compared with tamoxifen group （46.2%） , it showed no statistical difference （ P =0.863 ）. The positive rate of bel-2 in celeeoxib group （42.9%） was lower than that in control group （83.3%, P=0.010）, and also lower than that in tamoxifen group （ 53.9% ） , but without statistical difference （ P = 0. 568）. CONCLUSION： Celecoxib could significantly reduce the incidence rate of DMBA- induced breast cancer in rats. Downregulation of bel-2 and VEGF may be the mechanisms underlying the chemopreventive effect.
出处
《第四军医大学学报》
CAS
北大核心
2007年第1期27-29,共3页
Journal of the Fourth Military Medical University
基金
陕西省科技计划项目(2003K10-G40)
