摘要
目的观察胆管结石患者胆汁对人胆管癌细胞QBC939生长的影响 ,探讨胆管结石与胆管癌发生、发展的关系。方法应用噻唑蓝比色法检测 2 0份胆管结石患者胆汁和 10份正常胆汁对QBC939增殖的影响 ,应用流式细胞仪测定细胞周期和凋亡。结果胆管结石患者胆汁与正常胆汁比较 ,明显促进QBC939细胞增殖 ,用胆管结石患者胆汁处理 4 8h的QBC939细胞增殖指数显著上升 (P <0 0 1) ,胆管结石胆汁组 (47%± 10 % )S期细胞比例比正常胆汁组 (2 3%± 3% )明显增高 (P <0 0 1) ,G0 /G1期细胞比例 (42 %± 8% )比正常胆汁组 (6 3%± 10 % )明显降低 (P <0 0 5 )。结论胆管结石患者胆汁具有潜在的促增殖活性 ,胆管结石与胆管癌的发生。
Objective To explore the effects of bile from patients with choledocholithiasis on the growth of human cholangiocarcinoma cell line QBC939 and the potential relation between choledocholithiasis and cholangiocarcinoma. MethodsWT5”BZ Choledocholithiasis bile (CB) and normal bile (NB) specimen were used for this study. The proliferative effects of bile were measured by methabenzthiazuron (MTT) assay and cell cycle and apoptosis were analyzed by flow cytometry. KG2Results CB significantly promoted the proliferation of QBC939 cells, QBC939 proliferative index increased significantly after treated with 1% CB for 48 h ( P <0 01). The percentage of S phase (47%±10%)increased remarkably ( P <0 01) compared with NB (23%±3%), and the percentage of G0/G1 phase(42%±8%) decreased remarkably ( P <0 05) compared with NB (63%±10%). ConclusionKG1 CB can promote the proliferation of human cholangiocarcinoma QBC939 cells.
出处
《中华普通外科杂志》
CSCD
北大核心
2003年第10期605-606,共2页
Chinese Journal of General Surgery
