摘要
目的 利用四氯化碳诱导的实验性肝纤维化大鼠 ,探讨肝组织炎症活动度、肝纤维化程度与PCNA和bcl 2蛋白表达之间的关系。方法 将SD大鼠 4 4只随机分成正常对照组和病理模型组 ,每组 2 2只。结果 (1)病理模型组实验大鼠肝组织肝纤维化程度和bcl 2阳性表达计分均明显高于正常对照组 ,有统计学意义 (P <0 .0 1) ;(2 )病理模型组实验大鼠肝组织炎症活动度和肝纤维化程度呈明显正相关 (r =0 .80 ,P <0 .0 1) ;而细胞增殖指数(PI)与炎症活动度 (r=- 0 .6 0 ,P <0 .0 5 )和肝纤维化程度 (r =- 0 .6 4 ,P <0 .0 5 )呈负相关。结论 随着炎症活动度和肝纤维化程度加重 ,细胞增殖能力明显受损 ,而bcl 2在肝纤维化中的确切作用有待于进一步探讨。
Objective: To explore the relationship among the severity of inflammation, liver fibrosis, bcl 2 protein and proliferating cell nuclear antigen index (PCNA index, PI) in rats with experimental liver fibrosis by carbon tetrachloride(CCl 4). Methods: The 44 SD rats were randomly divided into the hepatofibrosis model and the normal control group. Results: ⑴Compared with the normal control group, the liver fibrosis scorings and the positive expression scorings of bcl 2 protein in rats of the pathologic model group were significantly increased in the pathologic model group,(P<0.01). ⑵In the pathologic model group, the relation between SI and SF (r=0.80, P<0.01) was significantly positive; And PI was in a significant negative correlation with the severity of inflammation (r=-0.60, P<0.05) and fibrosis (r=-0.64, P<0.05). Conclusion: The hepatocyte proliferation is hypoactive when the inflammation and liver fibrosis are severe. And the function of bcl 2 in liver fibrosis needs to be further studied.
出处
《泰山医学院学报》
CAS
2002年第1期4-6,共3页
Journal of Taishan Medical College
基金
浙江省教委资助课题 (NO19990 3 60 )
关键词
肝硬化
发病机制
增殖细胞核抗原
BCL-2蛋白
免疫组织化学
liver cirrhosis/pathogenic mechanisms
bcl 2 protein
proliferating cell nuclear antigen
immunohistochemistry
