摘要
选用基质辅助激光解吸离子化飞行时间质谱技术研究不同激光强度对猪血管紧张肽 (AngiotensinⅠ ,AnⅠ )解吸离子化的百分率和一级结构的稳定性。结果表明 :激光强度与AnⅠ解吸离子化百分率有关 ,AnⅠ多聚体解吸离子化所需的激光强度高于单体。AnⅠ与海兔酸性多肽 (Aplysiaacidicpeptide,AP)和海兔胰岛素Cβ(AplysiainsulinCβ,AICβ)混合后 ,采用延迟引出技术和激光强度 2 3 0 0IU分析混合样品 ,该激光强度不影响对AnⅠ和它的多聚体解吸离子化 ,但对AICβ 影响较大 ,相当于丢失了一分子OH-。相同的激光强度对不同结构的多肽多聚体产生不同的解吸离子化百分率 ,优化激光强度是准确分析多肽结构信息关键因素之一。
The percentage of desorption ionization and the stability of primary structure of angiotensinⅠ(AnⅠ) were studied using different laser intensities with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.The results show the relation between both laser intensity and the percentage of desorption ionization.Moreover,the laser intensity for the desorption ionization of the angiotensinⅠ polymer was known to be higher than its monomer.Using a technology of delayed extraction,the results showed that intensity hardly affected desorption ionization of both AnⅠ and its polymer after angiotensinⅠ,Aplysia acidic peptide and Aplysia insulin C β had been mixed.However,this reaction process greatly affected the desorption ionization of Aplysia insulin C β which caused the possibility that the peptide might release one OH--like molecule.Under the same laser intensity,the peptide polymer with different structure produced a different percentage of desorption ionization,which suggests that the optimization of laser intensity is a key factor for analyzing peptide structure.
出处
《动物学杂志》
CAS
CSCD
北大核心
2003年第5期34-40,共7页
Chinese Journal of Zoology
基金
国家自然科学基金 (No.4 0 2 76 0 33)
厦门市科技项目基金
教育部海洋与环境重点实验基金资助
关键词
猪血管紧张肽
激光强度
质谱
多肽与混合多肽
解吸离子化
AngiotensinⅠ
Laser intensity
Mass spectrometry
Peptide and its mixture
Desorption ionization
