摘要
蓝斑背肛海兔(Notarcus Leachii cirrosusStimpson,NLCS)属于海洋软体动物.NLCS大脑神经节(cerebral ganglion,CG)富含高丰度的脂类物质,并干扰了三氯醋酸-丙酮法沉淀CG蛋白质.本实验的目的是找到一种适合提取、分离海兔CG蛋白的方法.经各种实验方法对比后,发现超速离心法能有效分离CG破碎液中的脂类化合物,提高双向凝胶电泳法分离BG蛋白质组的分辨率,大约可检测到450个蛋白质点.
The Aplysia （Notarcus Leachii cirrosus Stimpson, NLCS） is an oceanic mollusk. The cerebral ganglion （CG） in NLCS contains high abundant lipid compounds, indicating that the compounds interfere strongly the precipitation of CG proteins with trichloroacetic acid-acetone. The object of this experiment is to find a method of extracting and separating the proteins of CG in aplysia. After comparison of several methods, an overspread centrifugation is discovered to have capacity for removing the lipid compounds in the suspension of CG for proteomic studies. The proteome resolution in CG can be improved with a typical separating approach of 2D-PAGE, which can detect about 650 protein spots.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第B05期198-201,共4页
Journal of Xiamen University：Natural Science
基金
国家自然科学基金(30470372)
福建省自然科学基金(C0310006)资助
关键词
海兔
大脑神经节
蛋白质组
优化分离
Aplysia, cerebral ganglion, proteome, optimized separation
