摘要
研究了基体辅助激光解析飞行时间质谱法（ＭＡＬＤＩＴＯＦＭＳ）测定蛋白和糖蛋白的实验条件，实测了重组人白细胞介素２、肿瘤坏死因子、粒细胞巨噬细胞集落刺激因子、白细胞干扰素α２ｂ、白细胞干扰素α１、促红细胞生成素、钙调蛋白及其片段、大鼠脑型一氧化氮合成酶和天然牛精液蛋白提取物的分子量和纯度，鉴定了蛋白质混合物中各单个成分，结果表明ＭＡＬＤＩＴＯＦＭＳ可有效地研究基因工程蛋白质和天然蛋白提取物等生物大分子的质量。
The experimental conditions of matrixassisted laser desorption/ionization time of flight mass spectrometry (MALDITOF MS) in the analysis of proteins and glycoproteins were studied and were used to analyze the molecular weights and purity of nine recombinant bioactive proteins, such as interleukin 2, tumor necrosis factor α, granulocytemacrophage colony stimulating factor, interferon α2b, interferon α1, erythropoietin, calmodulin and its fragments, neural nitric oxide synthase, and a natural protein extracted from bovine semen. It was also applied to determine the objective protein and calmodulin in an unknown mixture. In addition, the single ingredient in protein mixtures was characterized by the MALDITOF MS. The results showed that the technique of MALDITOF MS can be employed to determine the quality of recombinant and natural proteins effectively and accurately.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第4期290-295,共6页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
关键词
基体辅助
激光解析
飞行时间
质谱法
蛋白质
Matrixassisted laser desorption/ionization time of flight mass spectrometry
Proteins
Molecular weight
