摘要
目的 研究极低频电磁场暴露对小鼠脑和肝组织c fosmRNA水平的影响。方法 将小鼠暴露于 5 0Hz、0 .2mT及 5 0Hz、6 .0mT的电磁场中 ,持续 2周或 4周 ;采用竞争性RT PCR方法检测小鼠脑和肝脏c fosmRNA水平。结果 5 0Hz、0 .2mT及 5 0Hz、6 .0mT电磁场暴露 2周后 ,小鼠脑组织c fosmRNA的水平上升为 ( 0 .0 178± 0 .0 0 76 )amol 12 0ngcDNA和 ( 0 .0 0 92± 0 .0 0 4 2 )amol 12 0ngcDNA ,与对照组 [( 0 .0 0 12± 0 .0 0 0 5 )amol 12 0ngcDNA]比较 ,差异有显著性 (P <0 .0 5 ) ;肝组织c fosmRNA的水平上升为 ( 0 .0 117± 0 .0 0 5 5 )amol 12 0ngcDNA和 ( 0 .0 14 8± 0 .0 16 2 )amol 12 0ngcDNA ,与对照组[( 0 .0 0 0 5± 0 .0 0 0 5 )amol 12 0ngcDNA]比较 ,差异有显著性 (P <0 .0 5 )。 0 .2mT和 6 .0mT电磁场暴露4周后 ,小鼠脑组织c fosmRNA的水平上升为 ( 0 .0 10 0± 0 .0 0 5 4 )amol 12 0ngcDNA和 ( 0 .0 198±0 .0 0 79)amol 12 0ngcDNA ,与对照组 [( 0 .0 0 15± 0 .0 0 0 8)amol 12 0ngcDNA]比较 ,差异有显著性 (P <0 .0 5 ) ;肝组织c fosmRNA的水平分别上升为 ( 0 .0 173± 0 .0 12 2 )amol 12 0ngcDNA和 ( 0 .0 133±0 .0 0 90 )amol 12 0ngcDNA ,而对照组无表达。结论 5 0Hz电磁?
Objective To study the effects of extremely low frequency electromagnetic fields(ELF EMFs) on c fos gene expression in mouse brain and liver tissues. Methods Mice were exposed to 50 Hz sinusoidal 0.2 mT or 6.0 mT electromagnetic field for 2 weeks or 4 weeks.Competitive RT PCR method was used to measure c fos mRNA level. Results After exposure to 0.2 mT or 6.0 mT field for 2 weeks,c fos mRNA levels in brain tissue [(0.017 8 ±0.007 6)amol/120 ng cDNA and (0.009 2±0.004 2)amol/120 ng cDNA respectively] were higher than that of control level [(0.001 2±0.000 5)amol/120 ng cDNA](P<0.05).In liver tissue c fos mRNA levels[(0.011 7±0.005 5)amol/120 ng cDNA and (0.014 8 ±0.016 2)amol/120 ng cDNA respectively] were also higher than that of control level[(0.000 5±0.000 5)amol/120 ng cDNA](P<0.05).After exposure to 0.2 mT or 6.0 mT field for 4 weeks,c fos mRNA levels in brain tissue[(0.010 0±0.005 4)amol/120 ng cDNA and (0.019 8 ±0.007 9)amol/120 ng cDNA respectively] were higher than that of control level[(0.001 5± 0.000 8) amol/120 ng cDNA ](P<0.05).In liver tissue the exposure induced much higher expression level[(0.017 3±0.012 2) amol/120 ng cDNA and (0.013 3±0.009 0)amol/120 ng cDNA respectively] while no expression was found in the control. Conclusion Exposure to 50 Hz electromagnetic fields may induce up regulation of c fos transcription in mouse brain and liver tissue.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2003年第5期335-338,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
上海市生态学重点学科资助
