摘要
目的探讨不同强度的交变磁场辐照对人舌鳞癌细胞(TCa8113)增生的影响。方法不同强度的交变磁场(5mT、8mT、11mT)对人舌鳞癌细胞进行连续3天的辐照刺激,每天1h,通过MTT法和流式细胞术检测细胞的增生、凋亡和细胞周期的变化。对照组(假实验组)采用相同的实验条件和作用时间但不加磁场辐照。结果MTT法检测实验组与对照组辐照后24h、48h、72h的OD值,通过SPSS软件分析显示实验组比对照组的OD值显著降低(P<0.01)。流式细胞术检测电磁场组与对照组细胞凋亡率无差异。但细胞周期有显著改变(0.5mT)。结论不同强度的交变磁场辐照对人舌鳞癌细胞增生有明显抑制作用,并可阻滞细胞周期,但对舌鳞癌细胞的凋亡未发现明显影响。
Objective To study the effects of alternating magnetic field with different intensities on the proliferation of human squamous tongue cancer cells in vitro. Methods Viable cells with the OD value cell and flow eytometry were revealed through MTT assay to evaluate the proliferation and apoptosis and cell cycle respectively after the cells were exposed to electromagnetic fields of different intensity （5mT,8mT, 11mT） once per day lasting 1 hour for 3 days. The sham - exposure controls were correspondingly established. Results We compared the electromagnetic field groups with the normal groups by MTT assay after 24,48,72 hours. By analyzing the data in SPSS sta- tistical software , we found that the OD value of electromagnetic field groups was significantly less than that of the control groups （P 〈 0.01 ） . The rates of apoptosis cells by flow eytometry revealed that EMF groups had no change as compared with control groups. But the cell cycle displayed significant chang at 0.5mT . Conclusion The cells displayed significant changes with obvious Tca8113 cell proliferation inhibition and hold - up cell cycle after being exposed to alternating magnetic field of different intensity. But human squamous tongue cancer ceils could not be induced to apoptosis.
出处
《医学研究杂志》
2009年第8期32-36,共5页
Journal of Medical Research
关键词
人舌鳞癌细胞
交变磁场
凋亡
细胞周期
增生
Human squamous tongue cancer cell
Alternating magnetic field
Apoptosis
Cell cycle
Proliferation
