摘要
目的:探讨鼻咽癌患者血清Epstein-Barr病毒(EBV)DNA片段检出率及其和EBV衣壳抗原IgA类抗体(VCA/IgA)的关系。方法:聚合酶链反应(PCR)加限制性内切酶酶切技术检测疱疹病毒的DNA,酶免疫组化技术检测EBV VCA/IgA抗体。结果:33例鼻咽癌患者中,抗体阳性率88％,DNA阳性率55％;对照组120例,仅1例检出EBV DNA片段。鼻咽癌患者中EBV DNA片段的检出率显著性高于对照组,但EBV DNA片段的检出率低于EBV VCA/IgA抗体的检出率。结论:进一步证实EBV与鼻咽癌有密切关系;EBV DNA片段及EBV VCA/IgA抗体检测,有利于鼻咽癌的辅助诊断。
Objective: To explore the relationship of serum EBV DNA fragment and EBV VCA/IgA antibody titer in nasopharyngeal carcinoma (NPC) patients. Methods: PCR-restriction fragment length polymorphism was used to detect the EBV DNA and enzyme immunohistochemistry was used to measure the antibody titer. Results: Antibody positive rate was 88% in NPC group. DNA positive rates were 55% and 0. 83% in patients and control group respectively. Conclusions: The positive rate of EBV DNA fragment was much higher in NPC patient group than that of controls.but there was less sensitivi-ty of DNA detection than antibody detection in NPC group.
出处
《贵阳医学院学报》
CAS
2003年第5期386-388,共3页
Journal of Guiyang Medical College
基金
贵州省教育委员会基金(1996)
