摘要
目的探讨血浆EB病毒DNA、血清细胞角蛋白19片段21-1(CYFRA21-1)和EB病毒壳抗原IgA(VCA-IgA)含量在监测放疗后鼻咽癌复发和转移中的临床意义。方法分别采用荧光定量PCR法、电化学发光法和免疫酶法检测62例鼻咽癌患者放疗前、后血浆EBV DNA、血清CYFRA21-1和VCA-IgA含量。结果放疗前组阳性率显著高于放疗后缓解组(P<0.01),与复发或转移组比较无显著性差异(P>0.05);复发或转移组与放疗后缓解组相比较有高度显著性差异(P<0.01)。EBV DNA、CYFRA21-1、VCA-IgA检测的假阴性率分别为11.76%(2/17)、47.06%(8/17)、5.88%(1/17)。远处转移患者中有2例血浆EBV DNA阴性,其血清CYFRA21-1和VCA-IgA的检测为阳性。结论血浆EBV DNA、血清CYFRA21-1和VCA-IgA的联合检测是监测放疗后鼻咽癌患者复发、转移的有效指标。
OBJECTIVE To evaluate the clinical significance of quantitative analysis of EBV DNA in plasma,CYFRA21-1 and VCA-IgA in serum of nasopharyngeal carcinoma patients.METHODS EBV DNA in plasma,CRFRA21-1 and VCA-IgA in serum of 62 patients were analyzed using RealTime PCR technique,electrochemiluminescence immunoassay（ECI）,and immunoenzyme assay respectively.And these were compared among clinical remission,recurrent or metastasis groups.RESULTS The positive rate of preoperative radiotherapy group was significantly higher than clinical remission group（P0.01）.However,There was significant difference between recurrent or metastasis group and clinical remission group（P 0.01）.The false negative rate of the detection of Plasma EBV DNA,Serum CYFRA21-1 and VCA-IgA were 11.76%（2/17）,47.06%（8/17）and 5.88%（1/17）respectively.Two cases with metastasis,EBV DNA in plasma were negative,CYFRA21-1 and VCA-IgA in serum were positive.CONCLUSION The detection of Plasma EBV DNA,Serum CYFRA21-1 and VCA-IgA is useful for surveillance of nasopharynged carcinoma patients after radiotherapy.The simultaneous combination tests can increase the advantages to each other.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2010年第3期125-127,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
湖南省教育厅科研项目(09C046)
邵阳市科技计划项目(Z0904)联合资助
