摘要
目的 观察双氢青蒿素防治大鼠卡氏肺孢子虫肺炎 (PCP)的效果。方法 经皮下注射醋酸可的松诱导大鼠发生PCP。将受试大鼠随机分为实验组和对照组 ,双氢青蒿素防治方案分为小剂量长程预防、小剂量长程治疗及大剂量短程治疗三种。用肺重 /体重、肺虫荷量及肺泡卡氏肺孢子虫 (Pc)感染率 3项指标考核防治效果。结果 抽样检查证实大鼠已成功诱导PCP ,均为重度感染。方差分析及卡方检验显示 ,用双氢青蒿素防治的三组大鼠的三项指标均明显低于两个未防治对照组 (P <0 0 5或 0 0 1) ,与甲氧磺胺嘧啶 -磺胺甲基异恶唑 (TMP -SMZ)标准剂量治疗对照 (G)组无显著性差异 (P >0 0 5 )。结论 双氢青蒿素能有效防治大鼠的PCP ,效果与标准剂量TMP -SMZ接近。
Objective To estimate the ettect of dihydroarteminsinin(DHA)on the prophylaxis and therapy of Pneumocystis carinii pneumonia(PCP). Methods To develop PCP animal model,female Wistar rats were immunosuppressed by subcutaneous injection of 25mg cortisone acetate twice a week for 6 weeks. The rats were randomly divided into 7 groups:group A was normal control;group B and C were controls received no drug for prophylaxis or therapy;group D was given DHA at a dose of 30mg/kg·d for three weeks from week 4 to week 6,group E was given DHA at a dose of 30mg/kg·d for two weeks,group F was given DHA at a dose of 60mg/kg·d for five days,group G was given TMP-SMZ at a dose of 50mg/250mg/kg·d for five days. The efficiency of DHA on the prophylaxis and therapy of PCP was estimated by change of the ratio of lung weight to body weight and the burden ofPneumonia carinii(Pc). Results Fourteen rats were randomly killed at the end of the 6th week for the etiologic and pathologic examination. All of the rats were seriously infected by Pc. much lower mean ratio of lung weight to body weight,mean count of Pc cyst/mg of lung and moderate or serious infection rates of pulmonary alveoli were found in Group D,E and F received DHA than that of the control group B and C received no drug for the prophylaxis and therapy(P<0.05,or 0.01). But there were no significant difference among the 4 groups from D to G. Conclusions DHA could be used for the prophylaxis and therapy of PCP in the rat models,and its therapeutic efficacy was similar to that of standard dose TMP-SMZ.
出处
《临床和实验医学杂志》
2002年第1期33-36,共4页
Journal of Clinical and Experimental Medicine
